Anti-ZKSCAN3抗体(ab96731)
Key features and details
- Rabbit polyclonal to ZKSCAN3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ZKSCAN3抗体
参阅全部 ZKSCAN3 一抗 -
描述
兔多克隆抗体to ZKSCAN3 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-364 of Human ZNF306 (NP_077819).
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阳性对照
- WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji cells ICC/IF: HeLa cell
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96731于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 61 kDa.
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| ICC/IF |
1/100 - 1/200.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 61 kDa. |
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ICC/IF
1/100 - 1/200. |
靶标
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功能
Acts as a transcriptional regulator. Binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3'. Associates with chromatin at the ITGB4 and VEGF promoters. Activates the transcription of genes associated with colon cancer progression. -
组织特异性
Expressed in colorectal cancers (at protein level). -
序列相似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 7 C2H2-type zinc fingers.
Contains 1 KRAB domain.
Contains 1 SCAN box domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 80317 Human
- Omim: 612791 Human
- SwissProt: Q9BRR0 Human
- Unigene: 485004 Human
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别名
- dJ874C20.1 antibody
- mszf35 antibody
- SCAN-KRAB-zinc finger protein antibody
see all
图片
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Western blot - Anti-ZKSCAN3 antibody (ab96731)All lanes : Anti-ZKSCAN3 antibody (ab96731) at 1/1000 dilution
Lane 1 : HeLa S3 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-ZKSCAN3 antibody (ab96731)Immunofluorescence analysis of ZNF306 in paraformaldehyde fixed HeLa, using ab96731 at a 1/200 dilution.
Lower image (2): merged with DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab96731 尚未被引用在任何文献中。