Anti-ZIP Kinase抗体(ab2057)
Key features and details
- Rabbit polyclonal to ZIP Kinase
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ZIP Kinase抗体
参阅全部 ZIP Kinase 一抗 -
描述
兔多克隆抗体to ZIP Kinase -
宿主
Rabbit -
特异性
It has no cross reactivity to DAP kinase. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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免疫原
Synthetic peptide corresponding to Human ZIP Kinase aa 250-350 (internal sequence). ZIP kinase antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase.
Database link: O43293 -
阳性对照
- HeLa whole cell lysate or Jurkat whole cell lysate.
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常规说明
Apoptosis inducing kinase.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| 应用 | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Human
|
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
|
| WB | (1) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
WB
Use at an assay dependent concentration. |
靶标
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功能
Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Ubiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus.
Autophosphorylated. Phosphorylated by ROCK1. -
细胞定位
Nucleus. Cytoplasm. Nucleus > PML body. Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Localizes to promyelocytic leukemia protein nuclear bodies (PML-NBs). Associates to centromeres from prophase to anaphase. - Information by UniProt
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数据库链接
- Entrez Gene: 1613 Human
- Omim: 603289 Human
- SwissProt: O43293 Human
- Unigene: 631844 Human
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别名
- DAP kinase 3 antibody
- DAP like kinase antibody
- DAP-like kinase antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-ZIP Kinase antibody (ab2057)Immunocytochemistry/ Immunofluorescence of Jurkat cells staining ZIP Kinase with ab2057 at 10µg/ml
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Western blot - Anti-ZIP Kinase antibody (ab2057)Western Blot of HeLa and Jurkat cell lysates labeling ZIP Kinase with Anti-ZIP Kinase antibody (ab2057) at 1µg/ml.
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Western blot - Anti-ZIP Kinase antibody (ab2057)All lanes : Anti-ZIP Kinase antibody (ab2057) at 1/500 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-ZIP Kinase antibody (ab2057)ab2057 at 10µg/ml staining of ZIP kinase in Jurkat cell by ICC/IF
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Immunocytochemistry/ Immunofluorescence - Anti-ZIP Kinase antibody (ab2057)Immunofluorescence of ZIPK in Jurkat cells using ab2057 at 10 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab2057 被引用在 3 文献中.
- Yuan S et al. Tumor-associated macrophages affect the biological behavior of lung adenocarcinoma A549 cells through the PI3K/AKT signaling pathway. Oncol Lett 18:1840-1846 (2019). PubMed: 31423252
- Puto LA & Reed JC Daxx represses RelB target promoters via DNA methyltransferase recruitment and DNA hypermethylation. Genes Dev 22:998-1010 (2008). PubMed: 18413714
- Oster B et al. Human herpesvirus 6B induces phosphorylation of p53 in its regulatory domain by a CK2- and p38-independent pathway. J Gen Virol 89:87-96 (2008). PubMed: 18089732