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Cell Biology Apoptosis Intracellular Kinases

Anti-ZIP Kinase抗体(ab2057)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (3)

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Immunocytochemistry/ Immunofluorescence - Anti-ZIP Kinase antibody (ab2057)
  • Western blot - Anti-ZIP Kinase antibody (ab2057)
  • Western blot - Anti-ZIP Kinase antibody (ab2057)
  • Immunocytochemistry/ Immunofluorescence - Anti-ZIP Kinase antibody (ab2057)
  • Immunocytochemistry/ Immunofluorescence - Anti-ZIP Kinase antibody (ab2057)

Key features and details

  • Rabbit polyclonal to ZIP Kinase
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-ZIP Kinase抗体
    参阅全部 ZIP Kinase 一抗
  • 描述

    兔多克隆抗体to ZIP Kinase
  • 宿主

    Rabbit
  • 特异性

    It has no cross reactivity to DAP kinase.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide corresponding to Human ZIP Kinase aa 250-350 (internal sequence). ZIP kinase antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase.
    Database link: O43293

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • HeLa whole cell lysate or Jurkat whole cell lysate.
  • 常规说明

    Apoptosis inducing kinase.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • Other
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Other
    • Cancer
    • Cell Death
    • Apoptosis
    • Other

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant human ZIP Kinase protein (ab63192)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
ICC/IF
Human
WB
Human
应用 Ab评论 说明
ICC/IF
Use a concentration of 10 µg/ml.
WB (1)
Use at an assay dependent concentration.
说明
ICC/IF
Use a concentration of 10 µg/ml.
WB
Use at an assay dependent concentration.

靶标

  • 功能

    Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.
  • 序列相似性

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰

    Ubiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus.
    Autophosphorylated. Phosphorylated by ROCK1.
  • 细胞定位

    Nucleus. Cytoplasm. Nucleus > PML body. Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Localizes to promyelocytic leukemia protein nuclear bodies (PML-NBs). Associates to centromeres from prophase to anaphase.
  • Target information above from: UniProt accession O43293 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1613 Human
    • Omim: 603289 Human
    • SwissProt: O43293 Human
    • Unigene: 631844 Human
    • 别名

      • DAP kinase 3 antibody
      • DAP like kinase antibody
      • DAP-like kinase antibody
      • Dapk 3 antibody
      • DAPK3 antibody
      • DAPK3_HUMAN antibody
      • Death associated kinase 3 antibody
      • Death associated protein kinase 3 antibody
      • Death-associated protein kinase 3 antibody
      • Dlk antibody
      • EC 2.7.11.1 antibody
      • FLJ36473 antibody
      • MYPT1 kinase antibody
      • ZIP antibody
      • ZIP kinase antibody
      • ZIP kinase isoform antibody
      • ZIP-kinase antibody
      • ZIPK antibody
      • zipper-interacting protein kinase antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-ZIP Kinase antibody (ab2057)
      Immunocytochemistry/ Immunofluorescence - Anti-ZIP Kinase antibody (ab2057)
      Immunocytochemistry/ Immunofluorescence of Jurkat cells staining ZIP Kinase with ab2057 at 10µg/ml
    • Western blot - Anti-ZIP Kinase antibody (ab2057)
      Western blot - Anti-ZIP Kinase antibody (ab2057)

      Western Blot of HeLa and Jurkat cell lysates labeling ZIP Kinase with Anti-ZIP Kinase antibody (ab2057) at 1µg/ml.

    • Western blot - Anti-ZIP Kinase antibody (ab2057)
      Western blot - Anti-ZIP Kinase antibody (ab2057)
      All lanes : Anti-ZIP Kinase antibody (ab2057) at 1/500 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : Jurkat whole cell lysate

      Observed band size: 40 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-ZIP Kinase antibody (ab2057)
      Immunocytochemistry/ Immunofluorescence - Anti-ZIP Kinase antibody (ab2057)

      ab2057 at 10µg/ml staining of ZIP kinase in Jurkat cell by ICC/IF

    • Immunocytochemistry/ Immunofluorescence - Anti-ZIP Kinase antibody (ab2057)
      Immunocytochemistry/ Immunofluorescence - Anti-ZIP Kinase antibody (ab2057)
      Immunofluorescence of ZIPK in Jurkat cells using ab2057 at 10 ug/ml.

    实验方案

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (3)

    发表研究结果有使用 ab2057?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2057 被引用在 3 文献中.

    • Yuan S  et al. Tumor-associated macrophages affect the biological behavior of lung adenocarcinoma A549 cells through the PI3K/AKT signaling pathway. Oncol Lett 18:1840-1846 (2019). PubMed: 31423252
    • Puto LA & Reed JC Daxx represses RelB target promoters via DNA methyltransferase recruitment and DNA hypermethylation. Genes Dev 22:998-1010 (2008). PubMed: 18413714
    • Oster B  et al. Human herpesvirus 6B induces phosphorylation of p53 in its regulatory domain by a CK2- and p38-independent pathway. J Gen Virol 89:87-96 (2008). PubMed: 18089732

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Western blot abreview for Anti-ZIP Kinase antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Heart)
    Gel Running Conditions
    Reduced Denaturing (12% acrylamide)
    Loading amount
    20 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

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    Verified customer

    提交于 Jul 27 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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