Key features and details
- Rabbit polyclonal to ZIP Kinase
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
参阅全部 ZIP Kinase 一抗
描述兔多克隆抗体to ZIP Kinase
特异性It has no cross reactivity to DAP kinase.
经测试应用适用于: ICC/IF, WBmore details
种属反应性与反应: Mouse, Rat, Human
Synthetic peptide corresponding to Human ZIP Kinase aa 279-298 (internal sequence). ZIP kinase antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase.
Database link: O43293
(Peptide available as
- HeLa whole cell lysate or Jurkat whole cell lysate.
常规说明Apoptosis inducing kinase.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab2057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use at an assay dependent concentration.|
功能Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.
序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Contains 1 protein kinase domain.
翻译后修饰Ubiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus.
Autophosphorylated. Phosphorylated by ROCK1.
细胞定位Nucleus. Cytoplasm. Nucleus > PML body. Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Localizes to promyelocytic leukemia protein nuclear bodies (PML-NBs). Associates to centromeres from prophase to anaphase.
- Information by UniProt
- DAP kinase 3 antibody
- DAP like kinase antibody
- DAP-like kinase antibody
Immunocytochemistry/ Immunofluorescence of Jurkat cells staining ZIP Kinase with ab2057 at 10µg/ml
Western Blot of HeLa and Jurkat cell lysates labeling ZIP Kinase with Anti-ZIP Kinase antibody (ab2057) at 1µg/ml.
All lanes : Anti-ZIP Kinase antibody (ab2057) at 1/500 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Observed band size: 40 kDa why is the actual band size different from the predicted?
ab2057 at 10µg/ml staining of ZIP kinase in Jurkat cell by ICC/IF
Immunofluorescence of ZIPK in Jurkat cells using ab2057 at 10 ug/ml.
ab2057 被引用在 3 文献中.
- Yuan S et al. Tumor-associated macrophages affect the biological behavior of lung adenocarcinoma A549 cells through the PI3K/AKT signaling pathway. Oncol Lett 18:1840-1846 (2019). PubMed: 31423252
- Puto LA & Reed JC Daxx represses RelB target promoters via DNA methyltransferase recruitment and DNA hypermethylation. Genes Dev 22:998-1010 (2008). PubMed: 18413714
- Oster B et al. Human herpesvirus 6B induces phosphorylation of p53 in its regulatory domain by a CK2- and p38-independent pathway. J Gen Virol 89:87-96 (2008). PubMed: 18089732