重组Anti-ZFP36L1 (phospho S92)抗体[EPR19926] (ab204922)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19926] to ZFP36L1 (phospho S92)
- Suitable for: Dot blot, WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-ZFP36L1 (phospho S92)抗体[EPR19926]
参阅全部 ZFP36L1 一抗 -
描述
兔单克隆抗体[EPR19926] to ZFP36L1 (phospho S92) -
宿主
Rabbit -
经测试应用
适用于: Dot blot, WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HEK-293T transfected with human ZFP36L1 expression vector containing a myc-His-tag®, whole cell lysate; Wild-type mouse CD4+ and CD8+ T cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (PMA) and 1mM Ionomycin for 3 hours, whole cell lysate. Dot blot: ZFP36L1 (phospho S92) peptide (aa87-97).
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19926 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab204922于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Dot blot |
1/1000.
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WB |
1/5000. Detects a band of approximately 36-47 kDa (predicted molecular weight: 36 kDa).
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说明 |
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Dot blot
1/1000. |
WB
1/5000. Detects a band of approximately 36-47 kDa (predicted molecular weight: 36 kDa). |
靶标
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功能
Probable regulatory protein involved in regulating the response to growth factors. -
序列相似性
Contains 2 C3H1-type zinc fingers. -
细胞定位
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 677 Human
- Entrez Gene: 12192 Mouse
- Omim: 601064 Human
- SwissProt: Q07352 Human
- SwissProt: P23950 Mouse
- Unigene: 707091 Human
- Unigene: 85155 Human
- Unigene: 235132 Mouse
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别名
- Berg36 antibody
- BRF1 antibody
- Butyrate response factor 1 antibody
see all
图片
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All lanes : Anti-ZFP36L1 (phospho S92) antibody [EPR19926] (ab204922) at 1/2500 dilution
Lane 1 : Unstimulated wild-type mouse CD4+ and CD8+ T cells, whole cell lysate (Untreated membrane)
Lane 2 : Wild-type mouse CD4+ and CD8+ T cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (PMA) and 1mM Ionomycin for 3 hours, whole cell lysate (Untreated membrane)
Lane 3 : Unstimulated ZFP36L1 knockout mouse CD4+ and CD8+ T cells, whole cell lysate (Untreated membrane)
Lane 4 : ZFP36L1 knockout mouse CD4+ and CD8+ T cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (PMA) and 1mM Ionomycin for 3 hours, whole cell lysate (Untreated membrane)
Lane 5 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate (Untreated membrane)
Lane 6 : Unstimulated wild-type mouse CD4+ and CD8+ T cells, whole cell lysate (Phosphatase treated membrane)
Lane 7 : Wild-type mouse CD4+ and CD8+ T cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (PMA) and 1mM Ionomycin for 3 hours, whole cell lysate (Phosphatase treated membrane)
Lane 8 : Unstimulated ZFP36L1 knockout mouse CD4+ and CD8+ T cells, whole cell lysate (Phosphatase treated membrane)
Lane 9 : ZFP36L1 knockout mouse CD4+ and CD8+ T cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (PMA) and 1mM Ionomycin for 3 hours, whole cell lysate (Phosphatase treated membrane)
Lane 10 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate, (Phosphatase treated membrane)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773); and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/15000 dilution
Predicted band size: 36 kDa
Observed band size: 36-47 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 3% NFDM/TBST.
Lysates from the ZFP36L1fl/fl CD4 cre KO mouse were kindly provided by Dr Fiamma Salerno, Turner Lab, Babraham Institute.
Lysates used for the WB were isolated by negative selection using biotinylated abs against Ter119, CD11b, CD11c, Gr1, CD19, B220, F4/80 and NK1.1 to leave a population of CD4 and CD8 positive T cells (the purity of the population was >95% as checked by flow).
Mouse anti-vinculin ab130007, used as the loading control.
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All lanes : Anti-ZFP36L1 (phospho S92) antibody [EPR19926] (ab204922) at 1/5000 dilution
Lane 1 : HEK-293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T transfected with human ZFP36L1 expression vector containing a myc-His-tag®, whole cell lysate
Lane 3 : HEK-293T transfected with human ZFP36L1 expression vector containing a myc-His-tag®, then treated with alkaline phosphatase for 1 hour on the membrane
Lane 4 : HEK-293T transfected with human ZFP36L1 S92A mutant expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 36 kDa
Observed band size: 36-47 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 2% BSA/TBST.
All plasmids were kindly provided by Dr Fiamma Salerno, Turner Lab, Babraham Institute.
The expression profile observed is consistent with what has been described in the literature (PMID: 17030608).
Exposure time: 8 seconds
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Dot blot analysis of ZFP36L1 (phospho S92) labeled with ab204922 at 1/1000 dilution.
Lane 1: ZFP36L1 (phospho S92) peptide (aa87-97).
Lane 2: ZFP36L1 non-phospho peptide (aa87-97).
Lane 3: ZFP36L1 (phospho S125) peptide (aa120-130).
Lane 4: ZFP36L1 non-phospho peptide (aa120-130).Blocking and dilution buffer: 5% NFDM/TBST.Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Exposure time: 3 minutes.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab204922 被引用在 1 文献中.
- Feng H et al. MALAT1 Induces Food Allergy by Promoting Release of IL-6 from Dendritic Cells and Suppressing the Immunomodulatory Function of Tregs. J Asthma Allergy 15:529-544 (2022). PubMed: 35515816