重组Anti-ZBTB7A抗体[EPR13178(B)] (ab175918)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13178(B)] to ZBTB7A
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ZBTB7A抗体[EPR13178(B)]
参阅全部 ZBTB7A 一抗 -
描述
兔单克隆抗体[EPR13178(B)] to ZBTB7A -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IF, IPmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human ZBTB7A aa 500 to the C-terminus. The exact sequence is proprietary.
Database link: O95365 -
阳性对照
- MCF-7, HepG2, K562, Hela cell lysates, human bladder carcinoma tissue, human tonsil tissue, MCF7 and Hela cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13178(B) -
同种型
IgG
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175918于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 61 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/500.
For unpurified use at 1/100 - 1/250. |
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IP |
1/10 - 1/100.
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说明 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 61 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/500. For unpurified use at 1/100 - 1/250. |
IP
1/10 - 1/100. |
靶标
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功能
Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals (By similarity). Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3'. -
组织特异性
Widely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In tonsil, expressed in squamous epithelium and germinal center lymphocytes (at protein level). Up-regulated in a subset of lymphomas, as well as in a subset of breast, lung, colon, prostate and bladder carcinomas (at protein level). -
序列相似性
Contains 1 BTB (POZ) domain.
Contains 4 C2H2-type zinc fingers. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 51341 Human
- Omim: 605878 Human
- SwissProt: O95365 Human
- Unigene: 591384 Human
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别名
- DKFZp547O146 antibody
- Factor binding IST protein 1 antibody
- Factor that binds to inducer of short transcripts protein 1 antibody
see all
图片
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All lanes : Anti-ZBTB7A antibody [EPR13178(B)] (ab175918) at 1/10000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Hela cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 61 kDa -
Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) labeling ZBTB7A with purified ab175918 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
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Immunofluorescent staining of HeLa cells using labeling ZBTB7A with ab175918 at 1/100 dilution
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling ZBTB7A with ab175918 at 1/100 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue labeling ZBTB7A with ab175918 at 1/100 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate using labeling ZBTB7A with ab175918 at 1/10 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab175918 被引用在 8 文献中.
- Hao Y et al. Circular RNA Circ_0016760 Modulates Non-Small-Cell Lung Cancer Growth Through the miR-577/ZBTB7A Axis. Cancer Manag Res 12:5561-5574 (2020). PubMed: 32753969
- Yang H et al. FBI-1 enhanced the resistance of triple-negative breast cancer cells to chemotherapeutic agents via the miR-30c/PXR axis. Cell Death Dis 11:851 (2020). PubMed: 33051436
- Zhang K et al. Long Non-Coding RNA SNHG14 Impedes Viability, Migration and Invasion of Endometrial Carcinoma Cells Through Modulating miR-93-5p/ZBTB7A Axis. Cancer Manag Res 12:9515-9525 (2020). PubMed: 33061638
- Sun G et al. Upregulation of ZBTB7A exhibits a tumor suppressive role in gastric cancer cells. Mol Med Rep 17:2635-2641 (2018). PubMed: 29207095
- Lázaro S et al. Ablating all three retinoblastoma family members in mouse lung leads to neuroendocrine tumor formation. Oncotarget 8:4373-4386 (2017). PubMed: 27966456
- Shen Y et al. Negative feedback loop between ZBTB7A and TGF-ß in breast cancer. Oncol Lett 14:1403-1410 (2017). PubMed: 28789356
- de Vasconcellos JF et al. IGF2BP1 overexpression causes fetal-like hemoglobin expression patterns in cultured human adult erythroblasts. Proc Natl Acad Sci U S A 114:E5664-E5672 (2017). WB ; Human . PubMed: 28652347
- Hojo N et al. A Zbtb7a proto-oncogene as a novel target for miR-125a. Mol Carcinog 55:2001-2009 (2016). PubMed: 26713860