重组Anti-YTHDF2抗体[EPR20318] (ab220163)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20318] to YTHDF2
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-YTHDF2抗体[EPR20318]
参阅全部 YTHDF2 一抗 -
描述
兔单克隆抗体[EPR20318] to YTHDF2 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Wild-type mESC, HEK-293T, HT-1080 treated with 10µM MG-132 for 4 hours, K562, HEK-293 and HeLa whole cell lysate. Human brain, mouse and rat testis tissue lysate; GST-tagged human YTHDF2 recombinant protein. IP: HepG2 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20318 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab220163于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/1000. Predicted molecular weight: 62 kDa.
|
IP |
1/30.
|
说明 |
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WB
1/1000. Predicted molecular weight: 62 kDa. |
IP
1/30. |
靶标
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序列相似性
Contains 1 YTH domain. - Information by UniProt
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数据库链接
- Entrez Gene: 51441 Human
- Entrez Gene: 213541 Mouse
- Entrez Gene: 313053 Rat
- Omim: 610640 Human
- SwissProt: Q9Y5A9 Human
- SwissProt: Q91YT7 Mouse
- Unigene: 532286 Human
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别名
- 9430020E02Rik antibody
- CAHL antibody
- CLL associated antigen KW 14 antibody
see all
图片
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All lanes : Anti-YTHDF2 antibody [EPR20318] (ab220163) at 1/1000 dilution
Lane 1 : Wild-type mESC (mouse embryo stem cell) whole cell lysate
Lane 2 : YTHDF2 knockout mESC whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 62 kDa
Exposure time: 48 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
The wild-type and YTHDF2 knockout cell lysates were kindly provided by an anonymous collaborator.
ab220163 was shown to specifically react with YTHDF2 in wild-type mESC cells as signal was lost in YTHDF2 knockout cells. Wild-type and YTHDF2 knockout samples were subjected to SDS-PAGE. ab220163 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
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ab220163 Immunoprecipitating YTHDF2 in human HepG2 whole cell lysate. 10 µg of cell lysate was incubated with primary antibody (1/30). For western blotting ab220163 (1/1000) was used to confirm successful immunoprecipitation. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at (1/5000). For lane 3 Rabbit monoclonal IgG (ab172730) was used instead of ab220163.
In panel A, the lysate was stored at -80oC prior to the IP and incubation with the primary antibody was carried out overnight. In panel B, lysate was freshly made and used in the IP test immediately to minimize protein degradation. The incubation time was also shortened from overnight to 2h.
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All lanes : Anti-YTHDF2 antibody [EPR20318] (ab220163) at 1/5000 dilution
Lane 1 : Untreated HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate at 20 µg
Lane 2 : HT-1080 treated with 10µM MG-132 (ab141003) for 4 hours, whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 62 kDa
Exposure time: 103 secondsBlocking/diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-YTHDF2 antibody [EPR20318] (ab220163) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Mouse testis tissue lysate
Lane 3 : Rat testis tissue lysate
Lane 4 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
Lane 5 : K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 2-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 62 kDaBlocking/diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lane 1: 3 minutes; Lane 2: 92 seconds; Lane 3: 37 seconds; Lane 4: 8 seconds; Lane 5: 15 seconds.
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All lanes : Anti-YTHDF2 antibody [EPR20318] (ab220163) at 1/1000 dilution
Lane 1 : GST-tagged human YTHDF1 recombinant protein, 20ng
Lane 2 : GST-tagged human YTHDF2 recombinant protein, 20ng
Lane 3 : GST-tagged human YTHDF3 recombinant protein, 20ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 62 kDa
Exposure time: 3 secondsBlocking/diluting buffer and concentration: 5% NFDM/TBST.
The YTHDF recombinant proteins were kindly provided by an anonymous collaborator.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (43)
ab220163 被引用在 43 文献中.
- Zhao T et al. N6-methyladenosine plays a dual role in arsenic carcinogenesis by temporal-specific control of core target AKT1. J Hazard Mater 445:130468 (2023). IP ; Human . PubMed: 36444808
- Wang X et al. Transient regulation of RNA methylation in human hematopoietic stem cells promotes their homing and engraftment. Leukemia 37:453-464 (2023). RIP ; Human . PubMed: 36460765
- Jiao Y et al. The m6A reader YTHDC2 promotes SIRT3 expression by reducing the stabilization of KDM5B to improve mitochondrial metabolic reprogramming in diabetic peripheral neuropathy. Acta Diabetol 60:387-399 (2023). WB ; Rat . PubMed: 36574062
- Lin W et al. Tumor-intrinsic YTHDF1 drives immune evasion and resistance to immune checkpoint inhibitors via promoting MHC-I degradation. Nat Commun 14:265 (2023). WB ; Mouse . PubMed: 36650153
- Yang Y et al. O-GlcNAcylation of YTHDF2 promotes HBV-related hepatocellular carcinoma progression in an N6-methyladenosine-dependent manner. Signal Transduct Target Ther 8:63 (2023). WB, ICC, RIP ; Human . PubMed: 36765030