概述

  • 产品名称
  • 描述
    兔多克隆抗体to YIPF6
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Human YIPF6 aa 182-231 (C terminal).
    Sequence:

    MVRLFVVIVMFAWSIVASTALLADSQPPNRRALAVYPVFLFYFVISWMIL

  • 阳性对照
    • Fetal muscle lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab80442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Anti-YIPF6 antibody (ab80442) at 1 µg/ml + Fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

实验方案

文献

ab80442 has not yet been referenced specifically in any publications.

客户评价及客户问答

1-4 of 4 Q&A

Answer

Thank you for your reply. I am happy the information provided was helpful to you.


I have issued a credit note for the value of the antibody ab80442.


Your credit note ID is XXXXX.

The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.

(2) Held on the account for use against a future order.

(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice.

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Answer

I just wanted to update you with the last details provided by my lab colleagues regarding the use of this antibody in different cell lines and tissues.

They just confirmed they have unsuccessfully tested it on a few human cell lines (HepG2, Jurkat, MCF10, HeLa) and tissues (brain, lung, liver and placenta), but nothing gave as good a staining pattern as muscle tissue.

I hope this information is useful for you.

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Answer

Thank you for your reply.

We haven’t had any other complaints from customers using this antibody in WB or IHC, so unfortunately I am unable to inform you about its use in other labs.

My lab colleagues confirmed they have never used any other tissue or cell lines with this antibody before. However, according to its expression profile, based in the NCBI EST, this gen is predicted to be present in muscle:

http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.82719

Please let me know if I can help you further and if you wish to beneficiate from the Abpromise guarantee and receive a replacement or credit note.

Thank you very much for your comprehension.

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Answer

Thank you very much for taking the time to send us the questionnaire and the image attached.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

Reviewing the protocol, it seems perfectly fine for me. I would only suggest incubating the primary antibody at 4C overnight to optimise the epitope binding, as well as performing a non primary control, in order to check whether the 60KDa band could become from a non specific binding of the secondary, especially if in the Knock out cells you still observe this band.

Besides that, I am afraid there are no further tips to provide to improve the antibody’s performance. As the antibody is covered by our Abpromise guarantee, I can send you a free of charge replacement or a credit note in compensation.

Please let me know how you would like to proceed. If you have any other questions, do not hesitate to contact me back.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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