Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293E)
Loading amount
20 µg
Specification
HEK293E
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti Mouse
Host species: Goat
Clonality: Polyclonal
Host species: Goat
Clonality: Polyclonal
Dilution
1/5000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 19 kDa
Additional data
Abcam response
Please note that the recommended working dilution for WB is 1/150 (see PMID: 17103222).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 05 2007