Anti-XRCC1抗体(ab9147)
Key features and details
- Rabbit polyclonal to XRCC1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-XRCC1抗体
参阅全部 XRCC1 一抗 -
描述
兔多克隆抗体to XRCC1 -
宿主
Rabbit -
特异性
This antibody recognises human XRCC1 (x-ray cross complementing factor -1), a base excision repair protein. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Rat, Horse, Guinea pig, Hamster, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide (Human) conjugated to KLH - which represented aportion within the last 100 amino acids of the human X-ray Repair Cross Complementing Protein 1 (GenBank PID 11425942).
-
常规说明
Recently XRCC-1 has been shown to be physically associated with other DNA repair enzymes, including PARP, DNA ligase III and DNA polymerase beta.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Antibodies were affinity purified using thepeptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 70 kDa).
|
说明 |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 70 kDa). |
靶标
-
功能
Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. -
序列相似性
Contains 2 BRCT domains. -
翻译后修饰
Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
Sumoylated. -
细胞定位
Nucleus. Accumulates at sites of DNA damage. - Information by UniProt
-
数据库链接
- Entrez Gene: 616905 Cow
- Entrez Gene: 476447 Dog
- Entrez Gene: 7515 Human
- Entrez Gene: 84495 Rat
- Omim: 194360 Human
- SwissProt: P18887 Human
- SwissProt: Q9ESZ0 Rat
- Unigene: 98493 Human
see all -
别名
- DNA repair protein XRCC1 antibody
- RCC antibody
- X ray repair complementing defective repair in chinese hamster antibody
see all
图片
-
All lanes : Anti-XRCC1 antibody (ab9147) at 1 µg/ml
Lane 1 : Whole cell extract (50mcg) from mock treated HeLa cells.
Lane 2 : Whole cell extract (50mcg) from siRNA treated HeLa cells.
Predicted band size: 70 kDa
Observed band size: 76,90 kDa why is the actual band size different from the predicted?
Detection: ECL.
数据表及文件
-
SDS download
-
Datasheet download
文献 (15)
ab9147 被引用在 15 文献中.
- Ding X et al. The role of H3K9me2-regulated base excision repair genes in the repair of DNA damage induced by arsenic in HaCaT cells and the effects of Ginkgo biloba extract intervention. Environ Toxicol 36:850-860 (2021). PubMed: 33378118
- Flach KD et al. Endonuclease FEN1 Coregulates ERa Activity and Provides a Novel Drug Interface in Tamoxifen-Resistant Breast Cancer. Cancer Res 80:1914-1926 (2020). PubMed: 32193286
- Zhang Z et al. Triptolide interferes with XRCC1/PARP1-mediated DNA repair and confers sensitization of triple-negative breast cancer cells to cisplatin. Biomed Pharmacother 109:1541-1546 (2019). PubMed: 30551406
- Zhang LH et al. Up-regulated Wnt1-inducible signaling pathway protein 1 correlates with poor prognosis and drug resistance by reducing DNA repair in gastric cancer. World J Gastroenterol 25:5814-5825 (2019). PubMed: 31636474
- Sun C et al. Mobile phone signal exposure triggers a hormesis-like effect in Atm(+/+) and Atm(-/-) mouse embryonic fibroblasts. Sci Rep 6:37423 (2016). WB ; Mouse . PubMed: 27857169