概述

  • 产品名称
    Anti-XPG抗体[8H7]
    参阅全部 XPG 一抗
  • 描述
    小鼠单克隆抗体[8H7] to XPG
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant human XPG (Xeroderma Pigmentosum type G) protein produced in Baculovirus.

  • 表位
    8H7 binds between human XPG residues Ser 947 and Ala 1165.
  • 常规说明
    Works well on crude cell extract.

性能

应用

Our Abpromise guarantee covers the use of ab46 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/625 - 1/1250. Predicted molecular weight: 133 kDa.
IP Use at 2 µg/mg of lysate.

靶标

  • 功能
    Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Acts as a cofactor for a DNA glycosylase that removes oxidized pyrimidines from DNA. May also be involved in transcription-coupled repair of this kind of damage, in transcription by RNA polymerase II, and perhaps in other processes too.
  • 疾病相关
    Defects in ERCC5 are the cause of xeroderma pigmentosum complementation group G (XP-G) [MIM:278780]; also known as xeroderma pigmentosum VII (XP7). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-G patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities.
  • 序列相似性
    Belongs to the XPG/RAD2 endonuclease family. XPG subfamily.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COFS 3 antibody
    • COFS3 antibody
    • DNA excision repair protein ERCC 5 antibody
    • DNA excision repair protein ERCC-5 antibody
    • DNA excision repair protein ERCC5 antibody
    • DNA repair protein complementing XP G cells antibody
    • DNA repair protein complementing XP-G cells antibody
    • DNA repair protein complementing XPG cells antibody
    • ERCC 5 antibody
    • ERCC5 antibody
    • ERCC5_HUMAN antibody
    • ERCM 2 antibody
    • ERCM2 antibody
    • Excision repair cross complementation group 5 antibody
    • Excision Repair Cross Complementing Rodent Repair Deficiency antibody
    • Excision repair cross complementing rodent repair deficiency complementation group 5 antibody
    • Excision repair protein antibody
    • OTTHUMP00000064902 antibody
    • UVDR antibody
    • Xeroderma Pigmentosum Complementation Group G antibody
    • Xeroderma pigmentosum complementation group G protein antibody
    • Xeroderma pigmentosum group G complementing protein antibody
    • Xeroderma pigmentosum group G-complementing protein antibody
    • XPG antibody
    • XPG complementing protein antibody
    • XPGC antibody
    see all

文献

This product has been referenced in:
  • Trego KS  et al. The DNA repair endonuclease XPG interacts directly and functionally with the WRN helicase defective in Werner syndrome. Cell Cycle 10:1998-2007 (2011). Human . Read more (PubMed: 21558802) »
  • Igoucheva O  et al. Involvement of ERCC1/XPF and XPG in oligodeoxynucleotide-directed gene modification. Oligonucleotides 16:94-104 (2006). WB ; Human . Read more (PubMed: 16584298) »
See all 2 Publications for this product

客户评价及客户问答

1-8 of 8 Abreviews or Q&A

Question

Inquiry: Your e-mail adress technical@abcam.com is not working. So, here is the e-mail I wanted to send you.

Thank you for your prompt reply. You will find: 1. the answers to your questions 2. the pdf of the paper I was talking about 3. a powerpoint with photos of my experiment.
Thank you very much for your attention and I am looking forward hearing from you. As we are going to order this week an antibody for another protein, is it possible to send us at the same time few microliters of 2-3 other lots of XPG antibody to test.
Thank you.
Best regards,


Order Details
Antibody code: ab46
Lot number: GR20849-2
Purchase order number ######
or preferably Abcam order number:

General Information Antibody storage conditions (temperature/reconstitution etc) -20oC

Description of the problem (high background, low signal, non-specific satining etc.) non-specific local staining after local ultraviolet irradiation


Sample (Species/Tissue/Cell Type/Cell Line etc.) the MRC5 cell line

Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.) 2% paraformaldehyde

Antigen retrieval (Enzymatic method, Heat mediated technique etc.)

Permeabilization step 0.1% triton X100

Blocking conditions (Buffer/time period, Blocking agent etc.) PBS+0.15%glycine+0.5%BSA, 1h

Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) anti-XPG ab46 (abcam, mouse, dilution 1/500 in PBS+0.15%glycine+0.5%BSA)+ anti-PCNA ab18197 (abcam, rabbit, dilution 1/1000 in PBS+0.15%glycine+0.5%BSA) 2 hours, 3x 5 minutes wash step: PBS + 0.1% triton X100

Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) AlexaFluor 488 goat anti-mouse (invitrogen, goat, dilution 1/1000 in PBS+0.15%glycine+0.5%BSA)+ AlexaFluor 594 goat anti-rabbit (invitrogen, goat, dilution 1/1000 in PBS+0.15%glycine+0.5%BSA) 1 hour, 3x 5 minutes wash step: PBS + 0.1% triton X100, PBS, water before mounting with DAPI.

Detection method Zeiss microscope

Positive and negative controls used (please specify) We expect a co-localization with PCNA.

Optimization attempts (problem solving) dilutions 1/200, 1/500, 1/1000 +/- CSK100 before fixation with PAF 2% permeabilization with Methanol or Triton X-100

How many times have you tried the IHC? 4


Have you run a No Primary control? Yes No


Do you obtain the same results every time? Yes No What steps have you altered? Additional

Notes We would appreciate if you are also able to provide and image which woudl help us to assess the results

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Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

Reviewing this case, I would like to offer some suggestions to help optimize the results. I would also appreciate if you can confirm some further details:

1. I am sorry to confirm that as far as we are aware this antibody has not been tested in ICC-IF and regrettably usually we would not be able to provide our 6 month guarantee for this application. All tested applications and species covered by the guarantee are listed on the individual datasheets. I'm sorry the attachment of the publication you found did not come through and I would appreciate if you are able to try sending a this again so we have evidence that this clone has previously worked in this application.

2. Could you confirm how long was the fixation and permeabilisation step? I can suggest this should be 10 minutes only.

3. Could you confirm if you are fixing before or after the staining with the antibody? I can suggest to fix before antibody staining.

4. I can suggest trying the antibody on its own without the PCNA antibody double stain. It is often more effective to optimize antibodies individually before using them in multiple staining.

5. I can recommend to try overnight incubation at 4oC with the primary antibody. This can often provide more efficient and specific staining.

6. Try eliminating glycine from the buffers.

I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

Read More

Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I fully understand your concerns and I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch.

I would also like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB and IP and in human samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I would much appreciate if you are able to provide the following.

1. I am sorry we do not have access to all publications. I would appreciate if you are able to provide an attached copy of the publication you mention. It will be helpful for me to review the details in this.

2. I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

I would appreciate if you could also provide an image which would help us to assess the results.

Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.


Order Details
Antibody code: ab46

Lot number:

Purchase order number
or preferably Abcam order number:


General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, low signal, non-specific satining etc.)

Sample (Species/Tissue/Cell Type/Cell Line etc.)


Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)


Antigen retrieval (Enzymatic method, Heat mediated technique etc.)


Permeabilization step


Blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method


Positive and negative controls used (please specify)


Optimization attempts (problem solving)
How many times have you tried the IHC?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No


What steps have you altered?


Additional Notes


We would appreciate if you are also able to provide and image which woudl help us to assess the results

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa cell)
Total protein in input
700 µg
Specification
HeLa cell
Immuno-precipitation step
Protein A/G

Roberta Guglielmino

Verified customer

提交于 Apr 11 2007

Answer

Thank you for your patience. The antibody was used in the following publications which I hope will be of use to you: Evans E et al. 1997. EMBO J. 16:625-638. Constantinou A et al. 1999. J. Biol. Chem. 274:5637-5648. Araujo SJ et al. 2001. Mol. Cell. Biol. 21(7):2281-91. At this time this is all the information that I have. What type(s) of cell lystae did you use? At what dilution did you use ab46 at?

Read More

Answer

The MW of the the human XPG protein is 185 kDa and clone 8H7 binds between human XPG residues Ser 947 and Ala 1165. If you have any more questions, please contact us again.

Read More

Answer

I am still waiting for details regarding ab46 and will contact you when I receive them. For ab6264, the originator has informed me that the epitope has not been determined.

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Answer

This antibody has not been tested on paraffin sections.

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Question
Answer

This antibody has not yet been tested in mouse.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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