小鼠DLL1 ELISA试剂盒(ab208342)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 24 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠DLL1 ELISA试剂盒
参阅全部 DLL1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Mouse serum 5 2.8% 批次间 样品 n Mean SD CV% Mouse serum 3 7.2% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
24 pg/ml -
范围
62.5 pg/ml - 4000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 99 94% - 104% Serum 100 99% - 101% Hep Plasma 101 98% - 104% EDTA Plasma 103 102% - 105% Cit plasma 104 103% - 106% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Mouse
不与反应: Goat, Cow, Pig -
产品概述
Mouse DLL1 ELISA Kit (ab208342) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DLL1 protein in cell culture supernatant, serum, and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse DLL1 with 24 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
DLL1 (Delta-like protein 1) is a transmembrane glycoprotein that is needed during development for cell-to-cell communication in embryos and in the nervous system development. In adult tissues, DLL1 is involved in morphogenesis and remodeling and is overexpressed in several types of cancers.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Mouse DLL1 Capture Antibody 1 x 600µl 10X Mouse DLL1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse DLL1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. -
组织特异性
Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney. -
序列相似性
Contains 1 DSL domain.
Contains 8 EGF-like domains. -
翻译后修饰
Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation. -
细胞定位
Membrane. - Information by UniProt
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别名
- Delta
- Delta (Drosophila) like 1
- Delta like 1
see all -
数据库链接
- Entrez Gene: 13388 Mouse
- SwissProt: Q61483 Mouse
- Unigene: 4875 Mouse
相关产品
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Alternative Versions
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Related Products
图片
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of DLL1 were measured in duplicates, interpolated from the DLL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (citrate) 5%, plasma (heparin) 2.5% and spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DLL1 concentration was determined to be 26 ng/mL in serum, 26 ng/mL in plasma (citrate), 34 ng/mL in plasma (heparin) and 29 ng/mL in plasma (EDTA).
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
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