Anti-WSX-1抗体(ab5997)

Submit a review Q&A (1)References (2)
Western blot - Anti-WSX-1 antibody (ab5997)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WSX-1 antibody (ab5997)
  • Immunocytochemistry/ Immunofluorescence - Anti-WSX-1 antibody (ab5997)

Key features and details

  • Rabbit polyclonal to WSX-1
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-WSX-1抗体
    参阅全部 WSX-1 一抗

  • 描述

    兔多克隆抗体to WSX-1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ICC/IF, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide corresponding to Human WSX-1 aa 44-59 (N terminal).
    Sequence:

    GSAGPLQCYGVGPLGD

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • Human spleen tissue lysate.

  • 常规说明

    Protein previously labeled as TCCR.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5997于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 70 kDa.
ICC/IF
Use a concentration of 20 µg/ml.
IHC-P
Use a concentration of 10 µg/ml.
说明
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 70 kDa.
ICC/IF
Use a concentration of 20 µg/ml.
IHC-P
Use a concentration of 10 µg/ml.

靶标

  • 功能

    Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms.

  • 组织特异性

    Highly expressed in lymphoid tissues such as spleen, lymph nodes and peripheral blood leukocytes. Weakly expressed in other tissues examined including heart, brain, fetal and adult lung, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine, kidney and colon. In the lymphoid system, higher level expression in CD4+ T-cell subsets than in CD8+ T-cell subsets. Also weaker expression in CD19+ B-cells and monocytes.

  • 序列相似性

    Belongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 3 fibronectin type-III domains.

  • 结构域

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Class I cytokine receptor antibody
      • CRL1 antibody
      • Cytokine receptor-like 1 antibody
      • I27RA_HUMAN antibody
      • IL-27 receptor subunit alpha antibody
      • IL-27R subunit alpha antibody
      • IL-27R-alpha antibody
      • IL-27RA antibody
      • IL27R alpha antibody
      • IL27R antibody
      • Il27ra antibody
      • Interleukin 27 receptor alpha chain precursor antibody
      • Interleukin 27 receptor antibody
      • Interleukin-27 receptor subunit alpha antibody
      • Protein CRL1 antibody
      • T cell cytokine receptor type 1 antibody
      • TCCR antibody
      • Type 1 T cell cytokine receptor antibody
      • Type I T-cell cytokine receptor antibody
      • WSX-1 antibody
      • WSX1 antibody
      • ZcytoR1 antibody
      see all

    图片

    • Western blot - Anti-WSX-1 antibody (ab5997)
      Western blot - Anti-WSX-1 antibody (ab5997)

      Western blot analysis of WSX-1 expression in human spleen tissue lysates with ab5997 at 1 µg /ml. Western blot analysis of WSX-1 expression in human spleen tissue lysates with ab5997 at 1 µg /ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WSX-1 antibody (ab5997)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WSX-1 antibody (ab5997)

      ab5997 at 10µg/ml staining WSX-1 in human spleen tissue by IHC

    • Immunocytochemistry/ Immunofluorescence - Anti-WSX-1 antibody (ab5997)
      Immunocytochemistry/ Immunofluorescence - Anti-WSX-1 antibody (ab5997)

      Immunofluorescence of WSX-1 in A549 cells using ab5997 at 20 ug/ml.

    文献 (2)

    发表研究结果有使用 ab5997？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab5997 被引用在 2 文献中.

    • Seita J  et al. Interleukin-27 directly induces differentiation in hematopoietic stem cells. Blood 111:1903-12 (2008). PubMed: 18042804
    • Yoshimura T  et al. Two-sided roles of IL-27: induction of Th1 differentiation on naive CD4+ T cells versus suppression of proinflammatory cytokine production including IL-23-induced IL-17 on activated CD4+ T cells partially through STAT3-dependent mechanism. J Immunol 177:5377-85 (2006). PubMed: 17015723

    客户评价及客户问答

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    Question

    Hi, I used the Anti-TCCR antibody (ab5997) that my lab recently purchased from your company for Western blotting. I did not see a robust signal in the 70kDA region and the antibody also produced multiple bands on the membrane. It was difficult for me to distinguish the band for the antibody. I would like to ask technical support to help me trouble shoot this problem. Is it possible to send me positive controls to run with my samples?

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    Answer

    Thank you for contacting us. I am sorry to hear you are having problems with one of our products. Unfortunately, I am not available to send positive controls, but I can suggest using Human spleen tissue lysate with this antibody. We have in catalogue several lysates from Human Spleen Tissue if you are interested (see ab29699 and ab29689). https://www.abcam.com/Spleen-Human-Tissue-Lysate-adult-normal-tissue-ab29699.html https://www.abcam.com/Spleen-Human-Membrane-Lysate-adult-normal-tissue-ab29689.html However, I would be very happy to help you solve the problems experienced, and if no suggestions can be made to the protocol, and the antibody was purchased within the last 6 months, it is covered by our Abpromise guarantee, and therefore I can offer you a free of charge replacement or a credit note for it. I would appreciate if you could send me further details about the sample preparation such as the lysis buffer, the incubation process, the blocking conditions, etc... I send you a questionnaire attached to this email, which only takes about 5 minutes to be filled, and it will be very helpful to us to better understand why the antibody did not perform as stated in the datasheet. I would also appreciate if you could send us an image of the membrane, to better understand the problem. I look forward to receiving your reply. Please do not hesitate to contact us if you need any more advice or information.

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