Key features and details
- Rabbit polyclonal to WSX-1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.02% Sodium azide
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab5997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 70 kDa.|
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 10 µg/ml.|
功能Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms.
组织特异性Highly expressed in lymphoid tissues such as spleen, lymph nodes and peripheral blood leukocytes. Weakly expressed in other tissues examined including heart, brain, fetal and adult lung, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine, kidney and colon. In the lymphoid system, higher level expression in CD4+ T-cell subsets than in CD8+ T-cell subsets. Also weaker expression in CD19+ B-cells and monocytes.
序列相似性Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 3 fibronectin type-III domains.
结构域The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
- Information by UniProt
- Class I cytokine receptor antibody
- CRL1 antibody
- Cytokine receptor-like 1 antibody
Western blot analysis of WSX-1 expression in human spleen tissue lysates with ab5997 at 1 µg /ml. Western blot analysis of WSX-1 expression in human spleen tissue lysates with ab5997 at 1 µg /ml.
ab5997 at 10µg/ml staining WSX-1 in human spleen tissue by IHC
Immunofluorescence of WSX-1 in A549 cells using ab5997 at 20 ug/ml.
ab5997 被引用在 2 文献中.
- Seita J et al. Interleukin-27 directly induces differentiation in hematopoietic stem cells. Blood 111:1903-12 (2008). PubMed: 18042804
- Yoshimura T et al. Two-sided roles of IL-27: induction of Th1 differentiation on naive CD4+ T cells versus suppression of proinflammatory cytokine production including IL-23-induced IL-17 on activated CD4+ T cells partially through STAT3-dependent mechanism. J Immunol 177:5377-85 (2006). PubMed: 17015723