Anti-WSX-1抗体(ab5996)
Key features and details
- Rabbit polyclonal to WSX-1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-WSX-1抗体
参阅全部 WSX-1 一抗 -
描述
兔多克隆抗体to WSX-1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human WSX-1 aa 611-625 (C terminal).
Sequence:SGYEKHFLPTPEELG
(Peptide available asab39872) -
阳性对照
- Human spleen tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| IHC-P |
Use a concentration of 5 µg/ml.
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| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 70 kDa.
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| 说明 |
|---|
|
ICC/IF
Use a concentration of 20 µg/ml. |
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IHC-P
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 70 kDa. |
靶标
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功能
Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms. -
组织特异性
Highly expressed in lymphoid tissues such as spleen, lymph nodes and peripheral blood leukocytes. Weakly expressed in other tissues examined including heart, brain, fetal and adult lung, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine, kidney and colon. In the lymphoid system, higher level expression in CD4+ T-cell subsets than in CD8+ T-cell subsets. Also weaker expression in CD19+ B-cells and monocytes. -
序列相似性
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 3 fibronectin type-III domains. -
结构域
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 9466 Human
- Omim: 605350 Human
- SwissProt: Q6UWB1 Human
- Unigene: 132781 Human
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别名
- Class I cytokine receptor antibody
- CRL1 antibody
- Cytokine receptor-like 1 antibody
see all
图片
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Western blot - Anti-WSX-1 antibody (ab5996)Anti-WSX-1 antibody (ab5996) at 1 µg/ml + Human spleen tissue lysate
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WSX-1 antibody (ab5996)ab5996 at 5µg/ml staining WSX-1 in human spleen tissue by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-WSX-1 antibody (ab5996)Immunofluorescence of WSX-1 in Human Spleen cells using ab5996 at 20 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab5996 被引用在 2 文献中.
- Sugiyama N et al. Amelioration of human lupus-like phenotypes in MRL/lpr mice by overexpression of interleukin 27 receptor alpha (WSX-1). Ann Rheum Dis 67:1461-7 (2008). WB ; Mouse . PubMed: 18094002
- Takeda A et al. WSX-1 over-expression in CD4(+) T cells leads to hyperproliferation and cytokine hyperproduction in response to TCR stimulation. Int Immunol 17:889-97 (2005). PubMed: 15908443