为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair DNA Damage Response Other

Anti-WRNIP1/WHIP抗体(ab4731)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-WRNIP1/WHIP antibody (ab4731)

    Key features and details

    • Rabbit polyclonal to WRNIP1/WHIP
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    你可能会感兴趣

    二抗
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    查看更多相关产品

    概述

    • 产品名称

      Anti-WRNIP1/WHIP抗体
      参阅全部 WRNIP1/WHIP 一抗
    • 描述

      兔多克隆抗体to WRNIP1/WHIP
    • 宿主

      Rabbit
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Rat
    • 免疫原

      Synthetic peptide corresponding to Human WRNIP1/WHIP aa 107-120 conjugated to keyhole limpet haemocyanin.
      Sequence:

      SRESYDAPPTPSGAC

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

       This product was previously labelled as WRNIP1

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • DNA Damage Response
      • Other
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Synthesis
      • Other

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    应用

    Our Abpromise guarantee covers the use of ab4731 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB
  • 应用说明
    ELISA: 1/5000 - 1/20000.
    ICC/IF: Use at an assay dependent dilution (PMID 17888034).
    WB: 1/200. Detects a band of approximately 97 kDa (predicted molecular weight: 72 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能

      Functions as a modulator for initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. Has an intrinsic ATPase activity that functions as a sensor of DNA damage or of arrested replication forks and regulates the extent of DNA synthesis.
    • 组织特异性

      Ubiquitously expressed.
    • 序列相似性

      Belongs to the AAA ATPase family.
      Contains 1 UBZ-type zinc finger.
    • 翻译后修饰

      Sumoylated by SUMO1 and SUMO2/3.
    • 细胞定位

      Nucleus. Colocalizes with WRN in granular structures in the nucleus.
    • Target information above from: UniProt accession Q96S55 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 56897 Human
      • Entrez Gene: 78903 Mouse
      • Entrez Gene: 282835 Rat
      • Omim: 608196 Human
      • SwissProt: Q96S55 Human
      • SwissProt: Q91XU0 Mouse
      • SwissProt: Q8CG07 Rat
      • Unigene: 236828 Human
      • Unigene: 286680 Mouse
      • Unigene: 8783 Rat
      see all
    • 别名

      • ATPase WRNIP 1 antibody
      • ATPase WRNIP1 antibody
      • bA420G6.2 antibody
      • FLJ22526 antibody
      • Putative helicase RUVBL antibody
      • RP11 420G6.2 antibody
      • Werner helicasae interacting protein 1 antibody
      • Werner helicasae interacting protein antibody
      • Werner helicase interacting protein isoform 2 antibody
      • Werner helicase-interacting protein 1 antibody
      • WHIP antibody
      • WRIP1_HUMAN antibody
      • WRNIP 1 antibody
      • WRNIP antibody
      • Wrnip1 antibody
      see all

    图片

    • Western blot - Anti-WRNIP1/WHIP antibody (ab4731)
      Western blot - Anti-WRNIP1/WHIP antibody (ab4731)

      Western blot using ab4731 on HEK 293 lysate at 1/200.

       ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE.  Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected (indicated by an arrow). The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1/WHIP or post translational modification of the WRNIP1/WHIP protein.  The main band could be blocked using the immunising peptide.

      Western blot using ab4731 on HEK 293 lysate at 1/200.

      ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected (indicated by an arrow). The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1/WHIP or post translational modification of the WRNIP1/WHIP .

    实验方案

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
    • SDS
  • 文献 (2)

    发表研究结果有使用 ab4731?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4731 被引用在 2 文献中.

    • Kanu N  et al. RAD18, WRNIP1 and ATMIN promote ATM signalling in response to replication stress. Oncogene 35:4009-19 (2016). PubMed: 26549024
    • Mano Y  et al. Fibroblast growth factor receptor 1 oncogene partner as a novel prognostic biomarker and therapeutic target for lung cancer. Cancer Sci 98:1902-13 (2007). ICC/IF ; Human . PubMed: 17888034

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Western blot abreview for Anti-WRNIP1/WHIP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (8%)
    Loading amount
    10 µg
    Treatment
    lentivirus transduced for RNAi
    Specification
    293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 05 2009

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有