Anti-WRNIP1/WHIP抗体(ab4731)

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Western blot - Anti-WRNIP1/WHIP antibody (ab4731)

    Key features and details

    • Rabbit polyclonal to WRNIP1/WHIP
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-WRNIP1/WHIP抗体
      参阅全部 WRNIP1/WHIP 一抗

    • 描述

      兔多克隆抗体to WRNIP1/WHIP

    • 宿主

      Rabbit

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Rat

    • 免疫原

      Synthetic peptide corresponding to Human WRNIP1/WHIP aa 107-120 conjugated to keyhole limpet haemocyanin.
      Sequence:

      SRESYDAPPTPSGAC

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 常规说明

       This product was previously labelled as WRNIP1

       

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab4731于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (1)
    说明
  • 应用说明
    ELISA: 1/5000 - 1/20000.
    ICC/IF: Use at an assay dependent dilution (PMID 17888034).
    WB: 1/200. Detects a band of approximately 97 kDa (predicted molecular weight: 72 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • 靶标

    • 功能

      Functions as a modulator for initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. Has an intrinsic ATPase activity that functions as a sensor of DNA damage or of arrested replication forks and regulates the extent of DNA synthesis.

    • 组织特异性

      Ubiquitously expressed.

    • 序列相似性

      Belongs to the AAA ATPase family.
      Contains 1 UBZ-type zinc finger.

    • 翻译后修饰

      Sumoylated by SUMO1 and SUMO2/3.

    • 细胞定位

      Nucleus. Colocalizes with WRN in granular structures in the nucleus.
    • Information by UniProt

    • 数据库链接

      see all

    • 别名

      • ATPase WRNIP 1 antibody
      • ATPase WRNIP1 antibody
      • bA420G6.2 antibody
      • FLJ22526 antibody
      • Putative helicase RUVBL antibody
      • RP11 420G6.2 antibody
      • Werner helicasae interacting protein 1 antibody
      • Werner helicasae interacting protein antibody
      • Werner helicase interacting protein isoform 2 antibody
      • Werner helicase-interacting protein 1 antibody
      • WHIP antibody
      • WRIP1_HUMAN antibody
      • WRNIP 1 antibody
      • WRNIP antibody
      • Wrnip1 antibody
      see all

    图片

    • Western blot - Anti-WRNIP1/WHIP antibody (ab4731)
      Western blot - Anti-WRNIP1/WHIP antibody (ab4731)

      Western blot using ab4731 on HEK 293 lysate at 1/200.

       ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE.  Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected (indicated by an arrow). The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1/WHIP or post translational modification of the WRNIP1/WHIP protein.  The main band could be blocked using the immunising peptide.

      Western blot using ab4731 on HEK 293 lysate at 1/200.

      ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected (indicated by an arrow). The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1/WHIP or post translational modification of the WRNIP1/WHIP .

    文献 (2)

    发表研究结果有使用 ab4731？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab4731 被引用在 2 文献中.

    • Kanu N  et al. RAD18, WRNIP1 and ATMIN promote ATM signalling in response to replication stress. Oncogene 35:4009-19 (2016). PubMed: 26549024
    • Mano Y  et al. Fibroblast growth factor receptor 1 oncogene partner as a novel prognostic biomarker and therapeutic target for lung cancer. Cancer Sci 98:1902-13 (2007). ICC/IF ; Human . PubMed: 17888034

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    Western blot abreview for Anti-WRNIP1/WHIP antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (8%)
    Loading amount
    10 µg
    Treatment
    lentivirus transduced for RNAi
    Specification
    293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    提交于 Mar 05 2009

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