重组Anti-WIF1抗体[EPR9385] (ab155101)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9385] to WIF1
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-WIF1抗体[EPR9385]
参阅全部 WIF1 一抗 -
描述
兔单克隆抗体[EPR9385] to WIF1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human WIF1. The exact sequence is proprietary.
(Peptide available asab171766) -
阳性对照
- WB: HT29 and HeLa whole cell lysate (ab150035), human fetal heart and fetal lung tissue lysates, mouse and rat heart tissue lysates. IP: Human fetal lung tissue lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9385 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab155101于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/2000. Predicted molecular weight: 41 kDa.Can be blocked with Human WIF1 peptide (ab171766).
For unpurified use at 1/1000 - 1/10000. |
IP |
1/60.
For unpurified use at 1/10 - 1/100. |
说明 |
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WB
1/2000. Predicted molecular weight: 41 kDa.Can be blocked with Human WIF1 peptide (ab171766). For unpurified use at 1/1000 - 1/10000. |
IP
1/60. For unpurified use at 1/10 - 1/100. |
靶标
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功能
Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation. -
序列相似性
Contains 5 EGF-like domains.
Contains 1 WIF domain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 11197 Human
- Entrez Gene: 24117 Mouse
- Entrez Gene: 114557 Rat
- Omim: 605186 Human
- SwissProt: Q9Y5W5 Human
- SwissProt: Q9WUA1 Mouse
- SwissProt: Q6IN38 Rat
- Unigene: 284122 Human
see all -
别名
- WIF-1 antibody
- wif1 antibody
- WIF1_HUMAN antibody
- Wnt inhibitory factor 1 antibody
图片
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Anti-WIF1 antibody [EPR9385] (ab155101) at 1/2000 dilution (purified) + Human fetal heart tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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Anti-WIF1 antibody [EPR9385] (ab155101) at 1/5000 dilution (purified) + Human fetal lung tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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All lanes : Anti-WIF1 antibody [EPR9385] (ab155101) at 1/1000 dilution (purified)
Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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All lanes : Anti-WIF1 antibody [EPR9385] (ab155101) at 1/1000 dilution (unpurified)
Lane 1 : HT29 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal lung tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 41 kDa -
All lanes : Anti-WIF1 antibody [EPR9385] (ab155101) at 1/1000 dilution (unpurified)
Lanes 1-2 : pmHep3B whole cell lysate
Lanes 3-4 : Hep3B whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Alkaline Phosphatase conjugated goat anti-rabbit IgG polyclonal at 1/15000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 1 minute
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Purified ab155101 (1/60) immunoprecipitating WIF1 in human fetal lung whole cell lysate (Lane +). For western blotting an Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG (1/500) was used as the secondary antibody.
Lane (-): Rabbit monoclonal IgG (ab172730) instead of ab155101 in Human fetal lung whole cell lysate.
Blocking and diluting buffer: 5% NFDM/TBST.
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab155101 被引用在 16 文献中.
- Li K et al. miR-340 Promotes Retinoblastoma Cell Proliferation, Migration and Invasion Through Targeting WIF1. Onco Targets Ther 14:3635-3648 (2021). PubMed: 34113129
- Wei Y et al. miR-424-5p shuttled by bone marrow stem cells-derived exosomes attenuates osteogenesis via regulating WIF1-mediated Wnt/β-catenin axis. Aging (Albany NY) 13:17190-17201 (2021). PubMed: 34229300
- Zhou Y et al. Globular adiponectin inhibits osteoblastic differentiation of vascular smooth muscle cells through the PI3K/AKT and Wnt/β-catenin pathway. J Mol Histol 52:1067-1080 (2021). PubMed: 34398360
- Becker M et al. WIF1 Suppresses the Generation of Suprabasal Cells in Acanthotic Skin and Growth of Basal Cell Carcinomas upon Forced Overexpression. J Invest Dermatol 140:1556-1565.e11 (2020). PubMed: 31987884
- Niu J et al. Aberrant oligodendroglial-vascular interactions disrupt the blood-brain barrier, triggering CNS inflammation. Nat Neurosci 22:709-718 (2019). PubMed: 30988524