Key features and details
- Rabbit polyclonal to WDR21A
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab130417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
功能May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
通路Protein modification; protein ubiquitination.
序列相似性Contains 2 WD repeats.
- Information by UniProt
- DCAF4 antibody
- DCAF4_HUMAN antibody
- DDB1 and CUL4 associated factor 4 antibody
Immunocytochemistry/immunofluorescence analysis of MCF7 cells labelling WDR21A with ab130417 shows positive staining in the nucleus but excluded from the nucleoli.
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
Immunohistochemical analysis of Formalin-fixed, paraffin embedded Human uterus, pre-menopause tissue labelled with WDR21A antibody at 1/200 dilution.