Anti-WDR21A抗体(ab130417)
Key features and details
- Rabbit polyclonal to WDR21A
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-WDR21A抗体 -
描述
兔多克隆抗体to WDR21A -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment: TSSGTAGTSS VPELPGFYFD PEKKRYFRLL PGHNNCNPLT KESIRQKEME SKRLRLLQEE DRRK, corresponding to internal sequence amino acids 53-116 of Human WDR21A.
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阳性对照
- Human uterus, pre-menopause tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab130417于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 2 WD repeats. - Information by UniProt
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数据库链接
- Entrez Gene: 26094 Human
- SwissProt: Q8WV16 Human
- Unigene: 331491 Human
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别名
- DCAF4 antibody
- DCAF4_HUMAN antibody
- DDB1 and CUL4 associated factor 4 antibody
see all
图片
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Immunocytochemistry/immunofluorescence analysis of MCF7 cells labelling WDR21A with ab130417 shows positive staining in the nucleus but excluded from the nucleoli.
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Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil -
Immunohistochemical analysis of Formalin-fixed, paraffin embedded Human uterus, pre-menopause tissue labelled with WDR21A antibody at 1/200 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab130417 尚未被引用在任何文献中。