Application
Immunocytochemistry
Sample
Saccharomyces cerevisiae Cell
Fixative
Formaldehyde
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1%
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG Jackson IR
Host species: Donkey
Clonality: Monoclonal
Conjugation: Fluorescein
Host species: Donkey
Clonality: Monoclonal
Conjugation: Fluorescein
Additional data
Additional Notes
Spindle Pole Body is clearly stained and sometime tubulin cable are also stained, but is a non-specific staning.In blue : DAPI staining (nucleus and mitochondria)
In green : staining with anti VSV-G rabbit antibody (ab1874) showing the two SPB of yeast.
In green : staining with anti VSV-G rabbit antibody (ab1874) showing the two SPB of yeast.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Chesneau Laurent
Verified customer
提交于 Sep 19 2005