Anti-TRPV1抗体[BS397] - C-terminal (ab203103)
Key features and details
- Mouse monoclonal [BS397] to TRPV1 - C-terminal
- Suitable for: Flow Cyt (Intra), WB, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG2b
概述
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产品名称
Anti-TRPV1抗体[BS397] - C-terminal
参阅全部 TRPV1 一抗 -
描述
小鼠单克隆抗体[BS397] to TRPV1 - C-terminal -
宿主
Mouse -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Fr, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat
预测可用于: Guinea pig -
免疫原
Synthetic peptide within Rat TRPV1 aa 800 to the C-terminus (C terminal) (Cysteine residue). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O35433 -
阳性对照
- WB: PC-12 cell lysates; Mouse brain homogenate; forskolin and NGF stimulated 50B11 hybrid mouse and rat DRG cell lysates; NGF-stimulated PC-12 cell lysates. IHC-Fr: Mouse dorsal root ganglia. ICC/IF: C6 cells. Flow Cyt (Intra): PC-12 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Constituents: PBS, 3% Trehalose
also contains buffer salts -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
BS397 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab203103于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use 2µg for 106 cells.
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WB | (1) |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 95 kDa.
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IHC-Fr |
Use a concentration of 1 - 10 µg/ml.
Pre-absorption with immunogen obliterates positive staining. Frozen or PEG embedded tissues tested. |
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ICC/IF |
Use a concentration of 1 - 2 µg/ml.
Requires permeabilization step due to antigen epitope is intracellular |
说明 |
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Flow Cyt (Intra)
Use 2µg for 106 cells. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 95 kDa. |
IHC-Fr
Use a concentration of 1 - 10 µg/ml. Pre-absorption with immunogen obliterates positive staining. Frozen or PEG embedded tissues tested. |
ICC/IF
Use a concentration of 1 - 2 µg/ml. Requires permeabilization step due to antigen epitope is intracellular |
靶标
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功能
Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis. -
组织特异性
Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level). -
序列相似性
Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
Contains 6 ANK repeats. -
结构域
The association domain (AD) is necessary for self-association. -
翻译后修饰
Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity. -
细胞定位
Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 100135466 Guinea pig
- Entrez Gene: 193034 Mouse
- Entrez Gene: 83810 Rat
- SwissProt: Q6R5A3 Guinea pig
- SwissProt: Q704Y3 Mouse
- SwissProt: O35433 Rat
- Unigene: 447485 Mouse
- Unigene: 3073 Rat
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别名
- Capsaicin receptor antibody
- DKFZp434K0220 antibody
- osm 9 like TRP channel 1 antibody
see all
图片
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Immunohistochemical analysis of mouse dorsal root ganglia labelling TRPV1 with ab203103 at 10 µg/mL. Visualized with anti-mouse-Cy3 conjugate (red).
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Lane 1 : Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 2 µg/ml (overnight)
Lane 2 : Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 2 µg/ml ((pre-absorbed))
All lanes : Rat PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate
Lysates/proteins at 80 µg per lane.
Secondary
All lanes : Anti-mouse-HRP at 1/6000 dilution
Developed using the ECL technique.
Predicted band size: 95 kDaSDS-PAGE: denatured and reduced
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ab203103 staining TRPV1 in C6 (Rat glial tumor cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with 4% formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% horse serum. Samples were incubated with primary antibody (2 μg/ml, panel B) for 1 hour. A CF488A-conjugated Donkey anti-mouse polyclonal was used as the secondary antibody (5 μg/ml). Nuclei were stained with Hoechst dye (panel A) and panel C shows the merged image.
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Analysis of TRPV1 expression in PC-12 (Rat adrenal gland pheochromocytoma cell line) cell line by Flow Cyt (Intra) (black solid).
Cells were fixed and permeabilized in absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, then blocked with 1% BSA. Incubated with primary antibody for 30 minutes at RT. Secondary antibody used is a Goat anti-mouse PE labeled secondary antibody (1:100 dilution), 20 minutes in dark at room temperature.
Negative control: Non-specific Control IgG, clone X63 (black dashed).
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All lanes : Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 1 µg/ml ((4°C overnight))
Lane 1 : Forskolin + NGF
Lane 2 : Forskolin
Lane 3 : Control
Lane 4 : NGF control
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 95 kDa
Observed band size: 95-100 kDa why is the actual band size different from the predicted?(denatured and reduced)
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Lane 1 : Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 2 µg/ml
Lane 2 : Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 1 µg/ml
Lane 3 : Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 2 µg/ml (pre-absorbed)
All lanes : Mouse brain homogenate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Anti-mouse-HRP at 1/6000 dilution
Developed using the ECL technique.
Predicted band size: 95 kDaSDS-PAGE: denatured and reduced. ab203103 detects bands at 90-100 kDa and 180-200 kDa corresponding to TRPV1 monomer and dimer.
实验方案
数据表及文件
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Datasheet download
文献 (19)
ab203103 被引用在 19 文献中.
- Wen J et al. Role of transient receptor potential vanilloid-1 in behavioral thermoregulation of the Mongolian gerbil Meriones unguiculatus. Integr Zool 17:608-618 (2022). PubMed: 34498418
- Lazarini-Lopes W et al. Increased TRPV1 Channels and FosB Protein Expression Are Associated with Chronic Epileptic Seizures and Anxiogenic-like Behaviors in a Preclinical Model of Temporal Lobe Epilepsy. Biomedicines 10:N/A (2022). PubMed: 35203625
- Li X et al. Correlation of vascular change with TRPV1, TRPV4, and TRPA1 in a rat model of inferior gluteal artery perforator flap. Wound Repair Regen 30:365-375 (2022). PubMed: 35384152
- Zhu C et al. Angelica dahurica Extracts Attenuate CFA-Induced Inflammatory Pain via TRPV1 in Mice. Evid Based Complement Alternat Med 2022:4684830 (2022). PubMed: 35656472
- Yao E et al. OEA alleviates apoptosis in diabetic rats with myocardial ischemia/reperfusion injury by regulating the PI3K/Akt signaling pathway through activation of TRPV1. Front Pharmacol 13:964475 (2022). PubMed: 36452230