Anti-VR1抗体(ab6166)
Key features and details
- Rabbit polyclonal to VR1
- Suitable for: WB, IHC-FrFl
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-VR1抗体
参阅全部 VR1 一抗 -
描述
兔多克隆抗体to VR1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-FrFlmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide corresponding to Rat VR1 aa 819-838 (C terminal) conjugated to keyhole limpet haemocyanin (Glutaraldehyde).
Sequence:CGSLKPEDAEVFKDSMVPGEK
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常规说明
This antibody will stain a selected population of sensory neurons in the dorsal root ganglion. It is unsuitable for staining nerve fibres or terminals.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab6166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 94.976 kDa. | |
| IHC-FrFl | 1/100 - 1/2000. Use at 1/500– 1/2000 dilution for DAB staining and 1/100 – 1/500 dilution for immunofluorescence staining. |
靶标
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功能
Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis. -
组织特异性
Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level). -
序列相似性
Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
Contains 6 ANK repeats. -
结构域
The association domain (AD) is necessary for self-association. -
翻译后修饰
Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity. -
细胞定位
Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7442 Human
- Entrez Gene: 83810 Rat
- Omim: 602076 Human
- SwissProt: Q8NER1 Human
- SwissProt: O35433 Rat
- Unigene: 579217 Human
- Unigene: 655380 Human
- Unigene: 3073 Rat
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别名
- Capsaicin receptor antibody
- DKFZp434K0220 antibody
- osm 9 like TRP channel 1 antibody
see all
图片
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Western blot - Anti-VR1 antibody (ab6166)Anti-VR1 antibody (ab6166) at 1/1000 dilution + Mouse dorsal root ganglia at 20 µg
Predicted band size: 94.976 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
Immunohistochemistry - Free Floating - Anti-VR1 antibody (ab6166)Immunohistochemistry analysis of 4% paraformaldehyde fixed/free-floating mouse dorsal root ganglia detecting VR1 using ab6166 at a 1/500 dilution. 40x Magnification. Right: Negative control.
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Immunohistochemistry - Free Floating - Anti-VR1 antibody (ab6166)Immunohistochemistry analysis of 4% paraformaldehyde fixed/free-floating mouse dorsal root ganglia detecting VR1 using ab6166 at a 1/500 dilution. 40x Magnification. Right: Negative control.
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Immunohistochemistry - Free Floating - Anti-VR1 antibody (ab6166)Immunohistochemistry analysis of 4% paraformaldehyde fixed/free-floating mouse dorsal root ganglia detecting VR1 using ab6166 at a 1/500 dilution.
实验方案
数据表及文件
文献 (8)
ab6166 被引用在 8 文献中.
- Dos Anjos-Garcia T & Coimbra NC Opposing roles of dorsomedial hypothalamic CB1 and TRPV1 receptors in anandamide signaling during the panic-like response elicited in mice by Brazilian rainbow Boidae snakes. Psychopharmacology (Berl) N/A:N/A (2019). PubMed: 30694375
- Zhong B et al. TRPV1 protects renal ischemia-reperfusion injury in diet-induced obese mice by enhancing CGRP release and increasing renal blood flow. PeerJ 7:e6505 (2019). PubMed: 30834186
- Chen SJ et al. Protective Effects of Fucoxanthin on Ultraviolet B-Induced Corneal Denervation and Inflammatory Pain in a Rat Model. Mar Drugs 17:N/A (2019). PubMed: 30841522
- Recla JM et al. Genetic mapping in Diversity Outbred mice identifies a Trpa1 variant influencing late-phase formalin response. Pain 160:1740-1753 (2019). PubMed: 31335644
- Hu Q et al. TRPV1 Channel Contributes to the Behavioral Hypersensitivity in a Rat Model of Complex Regional Pain Syndrome Type 1. Front Pharmacol 10:453 (2019). PubMed: 31105572
- Li Y et al. Electroacupuncture Alleviates Paclitaxel-Induced Peripheral Neuropathic Pain in Rats via Suppressing TLR4 Signaling and TRPV1 Upregulation in Sensory Neurons. Int J Mol Sci 20:N/A (2019). PubMed: 31775332
- Grimaldi P et al. The endocannabinoid system and pivotal role of the CB2 receptor in mouse spermatogenesis. Proc Natl Acad Sci U S A 106:11131-6 (2009). WB ; Mouse . PubMed: 19541620
- Peier AM et al. A TRP channel that senses cold stimuli and menthol. Cell 108:705-15 (2002). IHC-Fr . PubMed: 11893340
