Anti-Von Willebrand Factor抗体(ab9378)
Key features and details
- Rabbit polyclonal to Von Willebrand Factor
- Suitable for: IHC-P, Flow Cyt, WB, IHC-Fr, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Von Willebrand Factor抗体
参阅全部 Von Willebrand Factor 一抗 -
描述
兔多克隆抗体to Von Willebrand Factor -
宿主
Rabbit -
经测试应用
适用于: IHC-P, Flow Cyt, WB, IHC-Fr, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Full length native protein (purified) corresponding to Human Von Willebrand Factor. Factor VIII related antigen isolated from human plasma.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.3 -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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IHC-P | (8) |
1/50 - 1/100.
|
Flow Cyt | (1) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 309 kDa).
|
IHC-Fr | (1) |
Use at an assay dependent concentration.
|
ICC/IF | (2) |
Use at an assay dependent concentration.
|
说明 |
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IHC-P
1/50 - 1/100. |
Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 309 kDa). |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. -
组织特异性
Plasma. -
疾病相关
Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII. -
序列相似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 4 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFA domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains. -
结构域
The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules. -
翻译后修饰
All cysteine residues are involved in intrachain or interchain disulfide bonds.
N- and O-glycosylated. -
细胞定位
Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules. - Information by UniProt
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数据库链接
- Entrez Gene: 7450 Human
- Entrez Gene: 22371 Mouse
- Omim: 613160 Human
- SwissProt: P04275 Human
- SwissProt: Q8CIZ8 Mouse
- Unigene: 440848 Human
- Unigene: 22339 Mouse
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别名
- Coagulation factor VIII antibody
- Coagulation factor VIII VWF antibody
- F8VWF antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Von Willebrand Factor with ab9378.
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Anti-Von Willebrand Factor antibody (ab9378) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 309 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 50 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Many bands of varying sizes can be seen on Western blots, perhaps indicating differential processing by ADAMTS13 and other enzymes -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining Von Willebrand Factor with ab9378.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab9378 被引用在 28 文献中.
- Lan G et al. Astrocytic VEGFA: An essential mediator in blood-brain-barrier disruption in Parkinson's disease. Glia 70:337-353 (2022). PubMed: 34713920
- Woods VMA et al. Targeting transgenic proteins to alpha granules for platelet-directed gene therapy. Mol Ther Nucleic Acids 27:774-786 (2022). PubMed: 35116189
- Guo S et al. Effects of diagnostic ultrasound with cRGD-microbubbles on simultaneous detection and treatment of atherosclerotic plaque in ApoE-/- mice. Front Cardiovasc Med 9:946557 (2022). PubMed: 35935617
- Zhang Q et al. Interrogation of the microenvironmental landscape in spinal ependymomas reveals dual functions of tumor-associated macrophages. Nat Commun 12:6867 (2021). PubMed: 34824203
- Sudhadevi T et al. Hyperoxia-induced S1P1 signaling reduced angiogenesis by suppression of TIE-2 leading to experimental bronchopulmonary dysplasia. Cell Biochem Biophys 79:561-573 (2021). PubMed: 34176100