Anti-Vitronectin/S-Protein抗体[A18] (ab23444)
Key features and details
- Mouse monoclonal [A18] to Vitronectin/S-Protein
- Suitable for: ELISA, IHC-P, Inhibition Assay, IHC-Fr
- Reacts with: Rabbit, Horse, Cow
- Isotype: IgG1
概述
-
产品名称
Anti-Vitronectin/S-Protein抗体[A18]
参阅全部 Vitronectin/S-Protein 一抗 -
描述
小鼠单克隆抗体[A18] to Vitronectin/S-Protein -
宿主
Mouse -
特异性
ab23444 is highly specific for Vitronectin/S-Protein. There is no evidence for cross reactivity with other connective tissue proteins (Fibronectin, Elastin, Collagen, Laminin).
-
经测试应用
适用于: ELISA, IHC-P, Inhibition Assay, IHC-Frmore details
不适用于: WB -
种属反应性
与反应: Rabbit, Horse, Cow
不与反应: Sheep, Human -
免疫原
Tissue, cells or virus corresponding to Cow Vitronectin/S-Protein. Lysed bovine corneal endothelial cells and extracellular matrix
-
常规说明
ab23444 can be used to probe vitronectin conformation.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
A18 -
骨髓瘤
Sp2/0 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23444于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration.
|
|
Inhibition Assay |
Use at an assay dependent concentration.
ab23444 inhibits integrin mediated cell adhesion to bovine vitronectin. |
|
IHC-Fr |
Use at an assay dependent concentration.
1/500 for PLP fixed samples. |
说明 |
---|
ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Inhibition Assay
Use at an assay dependent concentration. ab23444 inhibits integrin mediated cell adhesion to bovine vitronectin. |
IHC-Fr
Use at an assay dependent concentration. 1/500 for PLP fixed samples. |
靶标
-
功能
Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. -
组织特异性
Plasma. -
序列相似性
Contains 4 hemopexin repeats.
Contains 1 SMB (somatomedin-B) domain. -
结构域
The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin. -
翻译后修饰
Sulfated on 2 tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracellular medium. -
细胞定位
Secreted, extracellular space. - Information by UniProt
-
数据库链接
- Entrez Gene: 507525 Cow
- SwissProt: P22458 Rabbit
-
别名
- Complement S Protein antibody
- Epibolin antibody
- S Protein antibody
see all
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab23444 被引用在 1 文献中.
- Cámara-Torres M et al. Tuning Cell Behavior on 3D Scaffolds Fabricated by Atmospheric Plasma-Assisted Additive Manufacturing. ACS Appl Mater Interfaces 13:3631-3644 (2021). PubMed: 33448783