重组Anti-Vitamin D Receptor抗体[EPR4552] - ChIP Grade (ab109234)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4552] to Vitamin D Receptor - ChIP Grade
- Suitable for: WB, IP, ChIP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Vitamin D Receptor抗体[EPR4552] - ChIP Grade
参阅全部 Vitamin D Receptor 一抗 -
描述
兔单克隆抗体[EPR4552] to Vitamin D Receptor - ChIP Grade -
宿主
Rabbit -
经测试应用
适用于: WB, IP, ChIPmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, U-937, T-47D and SKBR-3 cell lysates; Rat and mouse kidney tissue lysates. ChIP: Chromatin prepared from T-47D cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4552 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109234于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
For unpurified use at 1/1000 - 1/10000. |
|
IP |
1/30.
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ChIP |
Use 5 µg for 25 µg of chromatin.
|
说明 |
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WB
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). For unpurified use at 1/1000 - 1/10000. |
IP
1/30. |
ChIP
Use 5 µg for 25 µg of chromatin. |
靶标
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功能
Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. -
疾病相关
Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets. -
序列相似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
结构域
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7421 Human
- Entrez Gene: 22337 Mouse
- Entrez Gene: 24873 Rat
- Omim: 601769 Human
- SwissProt: P11473 Human
- SwissProt: P48281 Mouse
- SwissProt: P13053 Rat
- Unigene: 524368 Human
see all -
别名
- 1 25 dihydroxyvitamin D3 receptor antibody
- 1 antibody
- 1,25 dihydroxyvitamin D3 receptor antibody
see all
图片
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All lanes : Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/1000 dilution
Lane 1 : Wild-type HeLa lysate
Lane 2 : Vitamin D Receptor knockout HeLa lysate
Lane 3 : U-937 lysate
Lane 4 : SH-SY5Y lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 48 kDaLanes 1-4: Merged signal (red and green). Green - ab109234 observed at 50 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109234 Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade was shown to specifically react with Vitamin D Receptor in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265430 (knockout cell lysate ab257796) was used. Wild-type and Vitamin D Receptor knockout samples were subjected to SDS-PAGE. ab109234 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Chromatin was prepared from T-47D cells according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 minutes. The ChIP was performed with 25 µg of chromatin, 5 µg of ab109234 (red), and 20 µL of protein A/G sepharose beads slurry (10 µL of sepharose A beads + 10 µL of sepharose G beads). 5 μg of rabbit normal IgG was added to the beads as a control sample (grey). The immunoprecipitated DNA was quantified by real time PCR (SYBR Green chemistry) with primers to c-Fos.
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Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/5000 dilution (purified) + HeLa cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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Lane 1 (input): T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate, 10 μg
Lane 2(+): T-47D whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109234 in T-47D whole cell lysateAb109234 immunoprecipitating Vitamin D receptor in T-47D whole cell lysates. Capture antibody was used at a 1/30 dilution (2 μg in 0.35 mg lysates). For western blotting, primary antibody was used as ab109234 at 1/1000 dilution (0.62 μg/mL). VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
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Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/5000 dilution (purified) + Mouse kidney tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/1000 dilution (purified) + Rat kidney tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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All lanes : Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/1000 dilution (unpurified)
Lane 1 : T47D cell lysate
Lane 2 : SKBR-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (27)
ab109234 被引用在 27 文献中.
- Zhou Y et al. The effects and possible mechanism of action of apolipoprotein M on the growth of breast cancer cells. Mol Biol Rep 49:1171-1179 (2022). PubMed: 34775573
- Gázquez A et al. Calcifediol During Pregnancy Improves Maternal and Fetal Availability of Vitamin D Compared to Vitamin D3 in Rats and Modifies Fetal Metabolism. Front Nutr 9:871632 (2022). PubMed: 35495908
- Wang Y & Jiang L Role of vitamin D-vitamin D receptor signaling on hyperoxia-induced bronchopulmonary dysplasia in neonatal rats. Pediatr Pulmonol 56:2335-2344 (2021). PubMed: 33878208
- Miura K et al. Vitamin D receptor is overexpressed in the duodenum of patients with irritable bowel syndrome. J Gastroenterol Hepatol 36:951-958 (2021). PubMed: 32839988
- Yang Y et al. Vitamin D protects glomerular mesangial cells from high glucose-induced injury by repressing JAK/STAT signaling. Int Urol Nephrol 53:1247-1254 (2021). PubMed: 33942213