Application
Western blot
Sample
Human Cell lysate - nuclear (Hodgkin lymphoma cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Hodgkin lymphoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Amersham ECL Rabbit IgG, HRP-linked whole Ab
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
OCI-Ly10 cells based on Real-Time PCR
Exposure
30 second(s)
Bands
Specific: 50 kDa Non-specific: many kDa
Positive control
TOLEDO, NUDUL1, OCI-Ly3 cells. Defined positive based on Real-Time PCR
Additional data
Additional Notes
Doesn't work for WB, many aspecific bands
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Dec 02 2020