Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human foreskin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 8.3
Permeabilization
Yes - 5 minutes w/ PBS-Triton 0.1%
Specification
Human foreskin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS-BSA 5%
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: anti-mouse Alexa 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Additional data
Additional Notes
Does not give any signals in these conditions.
Negative control correspond to secondary antibody only.
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jan 02 2020