Application
Western blot
Sample
Human Cell lysate - whole cell (Fibroblasts)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Fibroblasts
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 21°C
Other product details
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-mouse IgG (HRP)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
45 second(s)
Bands
Specific: 55 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 12 2022