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Neuroscience Cell Type Marker Neural Stem Cell marker
使用敲除细胞株进行验证重组RabMAb

重组Anti-Vimentin抗体[EPR3776] - Cytoskeleton Marker (ab92547)

  • Datasheet
  • SDS
Reviews (69)Q&A (1)References (734)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Western blot - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Flow Cytometry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Western blot - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Western blot - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Western blot - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Flow Cytometry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • OI-RD Scanning - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
  • Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3776] to Vimentin - Cytoskeleton Marker
  • Suitable for: ICC/IF, WB, Flow Cyt, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human, African green monkey

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概述

  • 产品名称

    Anti-Vimentin抗体[EPR3776] - Cytoskeleton Marker
    参阅全部 Vimentin 一抗
  • 描述

    兔单克隆抗体[EPR3776] to Vimentin - Cytoskeleton Marker
  • 宿主

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    African green monkey
    See all applications and species data
  • 免疫原

    Synthetic peptide within Human Vimentin aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P08670

  • 阳性对照

    • WB: HeLa, HEK293, Jurkat, A549, NIH3T3, PC12, HUVEC, Daudi, Caco-2 and COS-1 cell lysates; mouse and rat brain tissue lysates. IHC-P: Human kidney, colon, breast adenocarcinoma, cervical carcinoma and ovarian cancer tissues, mouse brain and kidney, E17 rat cheek and rat skin tissue sections; Rhesus monkey retina tissue. IHC-Fr: Mouse testis tissue. ICC/IF: HeLa, human adenocarcinoma, human schlemms canal endothelium and wild-type HAP1 cells. Flow Cyt: HeLa cells.
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 解离常数(KD)

    KD = 1.10 x 10 -10 M
    Learn more about KD
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EPR3776
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class III
    • Vimentin
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Cancer
    • Tumor biomarkers
    • Other
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Signal Transduction
    • Antibodies
    • vimentin

相关产品

  • Alternative Versions

    • Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab154207)
    • Alexa Fluor® 488 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab185030)
    • Anti-Vimentin antibody [EPR3776] - BSA and Azide free (ab193555)
    • HRP Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab194718)
    • Alexa Fluor® 647 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab194719)
    • Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab202504)
    • Alexa Fluor® 555 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab203428)
    • PE Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab209446)
    • Alexa Fluor® 405 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab210152)
    • Anti-Vimentin antibody [LN-6] (ab230171)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Vimentin protein (ab73843)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Human Vimentin Profiling ELISA Kit (ab173190)
    • Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    • Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab8069)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Mouse
Rat
Human
WB
Mouse
Rat
Human
African green monkey
All applications
Rhesus monkey
应用 Ab评论 说明
ICC/IF (16)
1/250 - 1/1000.
WB (11)
1/1000 - 1/5000. Predicted molecular weight: 54 kDa.
Flow Cyt (2)
1/50 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P (28)
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
ICC/IF
1/250 - 1/1000.
WB
1/1000 - 1/5000. Predicted molecular weight: 54 kDa.
Flow Cyt
1/50 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • 组织特异性

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • 疾病相关

    Cataract 30
  • 序列相似性

    Belongs to the intermediate filament family.
  • 结构域

    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • 翻译后修饰

    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • 细胞定位

    Cytoplasm.
  • Target information above from: UniProt accession P08670 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 7431 Human
    • Entrez Gene: 22352 Mouse
    • Entrez Gene: 81818 Rat
    • Omim: 193060 Human
    • SwissProt: P08670 Human
    • SwissProt: P20152 Mouse
    • SwissProt: P31000 Rat
    • Unigene: 455493 Human
    • Unigene: 691131 Human
    • Unigene: 268000 Mouse
    • Unigene: 2710 Rat
    see all
  • 形式

    Vimentin is found in connective tissue and in the cytoskeleton.
  • 别名

    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)

    Immunohistochemical staining of paraffin embedded mouse kidney with purified ab92547 at a working dilution of 1/250. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Western blot - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    All lanes : Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) Whole Cell Lysate
    Lane 2 : HEK293 (Human epithelial cell line from embryonic kidney) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole cell lysate
    Lane 4 : A549 (Human lung carcinoma cell line) Whole cell lysate
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 6 : PC12 (Rat adrenal gland pheochromocytoma cell line) Whole Cell Lysate
    Lane 7 : HUVEC (Human umbilical vein endothelial cell line) Whole cell lysate
    Lane 8 : A431 (Human epidermoid carcinoma cell line) Whole cell lysate
    Lane 9 : Daudi (Human Burkitt's lymphoma cell line) Whole cell lysate
    Lane 10 : Caco 2 (Human colorectal adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : IRDye® 800CW Goat Anti-Rabbit at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 54 kDa
    Observed band size: 53 kDa why is the actual band size different from the predicted?



    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using LI-COR® blocking buffer before being incubated with ab92547 overnight at 4°C. Antibody binding was detected using the IRDye® 800CW Goat Anti-Rabbit secondary at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Odyssey® CLx Imaging System.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)Image from Muniz-Talavera H and Schmidt JV., PLoS One. 2017;12(12):e0184957. Fig 3.; doi: 10.1371/journal.pone.0184957. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    JhylacZ/lacZ mice exhibit delayed radial glial to ependymal cell differentiation.

    Immunohistochemical analysis of P10 lateral ventricle coronal sections from Jhy+/+ (A, E) and JhylacZ/lacZ (I, M) mice for expression of Vimentin (pink, ab92547), Glast (green) and Acα-Tub (orange) in dorsal (A-D, I-L) and ventral (E-H, M-P) brain regions. Lower right panels (D, L, H, P) represent a higher magnification view of the merged image. In Jhy+/+, medial wall dorsal and ventral cells express the differentiated ependymal markers Vimentin (A, B, E, F) and Acα-Tub (A, D, E, H), but are negative for the radial glial marker Glast (A, C, E, G). In JhylacZ/lacZ brains, some dorsal cells remain positive for the undifferentiated marker Glast (I, K), while also expressing the differentiated markers Vimentin and Acα-Tub (I, J, L). JhylacZ/lacZ ventral cells express only Vimentin and Acα-Tub (M-P). The dotted line indicates the medial wall ependymal cells in (C, G, K, O). (Q-R) Graphical representation of the percentage of Glast(-)Vimentin(+)Acα-Tub(+) (black bar) and Glast(+)Vimentin(+)Acα-Tub(+) (grey bar) cells in dorsal (Q) and ventral (R) ependymal cells. MW, medial wall; LW, lateral wall; LV, lateral ventricle; * denotes p≤0.05. Scale bars: 50μm (A-P).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab92547 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)This image is courtesy of an anonymous Abreview

     

    Immunohistochemical staining of paraffin embedded paraformaldehyde fixed rhesus monkey retina tissue with ab92547 (green) at a working dilution of 1/200. The sample was incubaded with the primary antibody fro 20 hours, at 4°C in 2.5% serum. The secondary antibody used is a Goat anti-rabbit AlexaFluor 488 at 1/400. Heat mediated antigen retrieval was perfomed using citrate pH 6. Tissue was blocked with 5% serum for 1 hour 30 minutes at 25°C

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)

    IHC image of unpurified ab92547 staining Vimentin in human breast adenocarcinoma formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92547, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)

    ab92547 staining Vimentin in wild-type HAP1 cells (top panel) and VIM knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab92547 at 0.5μg/ml and ab195889 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)Image from Prestigiacomo V et al., PLoS One. 2017;12(6):e0179995. Fig 7.; doi: 10.1371/journal.pone.0179995.

     Immunostaining of formalin fixed paraffin embedded human microtissues after exposure to MTX, TAA and TGF-β1.

    Formalin fixed paraffin embedded slides of HepaRG/THP-1 macrophages/hTERT-HSC microtissues were stained with Hematoxylin & Eosin (H&E) and vimentin after 14 days of treatment with MTX, TAA and TGF-β1. Microtissues were fixed in 4% PFA and embedded in 2% agarose prior to paraffinization. Microtissues showed increase in the vimentin positive cells after MTX, TAA and TGF-β1 exposure. Vimentin stainings show proliferation of stellate cells and THP-1 macrophages in the microtissues, suggesting the onset of inflammation process.

    For full image see PMID 28665955.

  • Flow Cytometry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Flow Cytometry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Overlay histogram showing HAP1 wildtype (green line) and HAP1-VIM knockout cells (red line) stained with ab92547. The cells were fixed with  80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab92547, 0.5µg/ml) for 30 min at 22°C. The secondary antibody used was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/2000 dilution for 30 min at 22°C.
    A Rabbit IgG isotype control antibody  (ab172730) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-VIM knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
  • Western blot - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Western blot - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    All lanes : Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547) at 1/5000 dilution (purified)

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HEK293 (Human epithelial cell line from embryonic kidney) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Western blot - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    All lanes : Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547) at 1/5000 dilution (purified)

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Western blot - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547) at 1/20000 dilution (purified) + COS-1 (African green monkey kidney fibroblast-like cell line) cell lysate at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)

    Immunofluorescence staining of HeLa (human epithelial cell line from cervix adenocarcinoma) cells with purified ab92547 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody, used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) 1/1000, shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab92547 was used at a dilution of 1/500 followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at a dilution of 1/400.

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)

    ab92547 staining Vimentin in HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab92547 at 5μg/ml and ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 2 μg/ml (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.

    Negative controls: 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)

    Unpurified ab92547 staining Vimentin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab92547 at a working concentration of 5μg/ml and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 1/250 overnight at +4°C, followed by a further incubation at room temperature for 1h with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow Cytometry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Flow Cytometry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Overlay histogram showing HeLa cells fixed in 2% PFA and stained with purified ab92547 at a dilution of 1 in 50 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)

    Anti-vimentin (ab92547) staining in E17 rat cheek sections using immunohistochemistry (formaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/2000) for two hours at room temperature. A biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    Image courtesy of Mr Carl Hobbs, Kings College London.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)

    Anti-vimentin (ab92547) staining in adult mouse brain (the dentate gyrus region of the hippocampus) using immunohistochemistry (formaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/2000) for two hours at room temperature. A biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    Image courtesy of Mr Carl Hobbs, Kings College London.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)

    Anti-vimentin (ab92547) staining in human ovarian cancer tissue using immunohistochemistry (formaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/2000) for two hours at room temperature. A biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    Image courtesy of Mr Carl Hobbs, Kings College London.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)

    Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human cervical carcinoma tissue sections labeling Vimentin with ab92547.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)

    Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue sections labeling Vimentin with ab92547.

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)This image is courtesy of an Abreview submitted by Thomas Read

    Unpurified ab92547 staining vimentin in human Schlemms Canal Endothelium cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 0.2% and blocked with 10% serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/200 in DPBS) for 3 hours at 20°C. An undiluted Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)

    Fluorescent immunohistochemical analysis of paraffin-embedded human normal kidney tissue using unpurified ab92547. Green- Vimentin red-PI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)

    Fluorescent immunohistochemical analysis of paraffin-embedded human normal colon tissue using unpurified ab92547. Green- Vimentin red-PI

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)This image is courtesy of an anonymous Abreview.

    ab92547 staining Vimentin in rat skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% buffered normal formalin and blocked with 5% serum for 60 minutes at 21°C; antigen retrieval was by heat mediation in a 10mM Sodium citrate buffer. Samples were incubated with primary antibody (1/200 in blocking buffer) for 12 hours at 4°C. A Cy3®-conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)Image courtesy of an anonymous Abreview

    Immunofluorescence staining of NIH/3T3 (mouse embryo fibroblast cell line) whole cells with ab92547 at 1/1000 dilution in PBS, 1% BSA 0,1% Triton-X-100. The secondary antibody was Alexa Fluor­ 555 Donkey Anti-Rabbit IgG, used at a dilution of 1/1500. The cells were fixed in formaldehyde and permeabilized using 0.2% Triton X 100. Then blocked with 3% BSA for 30 minutes at room temperature.

    See Abreview

  • OI-RD Scanning - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    OI-RD Scanning - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)

    Equilibrium dissociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

  • Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
    Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)

实验方案

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (734)

发表研究结果有使用 ab92547?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab92547 被引用在 734 文献中.

  • Wang W  et al. Enterovirus A71 capsid protein VP1 increases blood-brain barrier permeability and virus receptor vimentin on the brain endothelial cells. J Neurovirol 26:84-94 (2020). PubMed: 31512144
  • Meyer M  et al. Mouse genetics identifies unique and overlapping functions of fibroblast growth factor receptors in keratinocytes. J Cell Mol Med 24:1774-1785 (2020). PubMed: 31830366
  • Li Q  et al. Silencing of synaptotagmin 13 inhibits tumor growth through suppressing proliferation and promoting apoptosis of colorectal cancer cells. Int J Mol Med 45:234-244 (2020). PubMed: 31939613
  • Gao L  et al. TNAP inhibition attenuates cardiac fibrosis induced by myocardial infarction through deactivating TGF-ß1/Smads and activating P53 signaling pathways. Cell Death Dis 11:44 (2020). PubMed: 31969558
  • Tan H  et al. Peimine inhibits the growth and motility of prostate cancer cells and induces apoptosis by disruption of intracellular calcium homeostasis through Ca2+ /CaMKII/JNK pathway. J Cell Biochem 121:81-92 (2020). PubMed: 31081133
View all Publications for this product

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1-10 of 16 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody [EPR3776]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Sample
Mouse Cell (NIH/3T3)
Specification
NIH/3T3
Permeabilization
Yes - 0.2% Triton-X-100
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

提交于 Aug 12 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody [EPR3776]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Schlemms Canal Endothelium)
Specification
Schlemms Canal Endothelium
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Read More

Dr. Thomas Read

Verified customer

提交于 Oct 31 2011

Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Mesenchymal cells)
Permeabilization
No
Specification
Mesenchymal cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Feb 10 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (embryonic intestinal cells)
Permeabilization
Yes - TritonX100 0.5%
Specification
embryonic intestinal cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Dec 16 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Permeabilization
Yes - 0.01% TritonX-100
Specification
HEK293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Jul 03 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat H9c2 cardiomyocytes)
Permeabilization
Yes - 0.01% TritonX-100
Specification
Rat H9c2 cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

提交于 Mar 18 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - 0.01% TritonX-100
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Mar 18 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Skin, dermis)
Permeabilization
No
Specification
Skin, dermis
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

提交于 Mar 20 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Trophoblast cell line)
Permeabilization
Yes - 0.1% triton X in PBS
Specification
Trophoblast cell line
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Methanol
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Ms. Lisa Campbell

Verified customer

提交于 Aug 22 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MDAMB231)
Permeabilization
Yes - 0.2% Triton x100
Specification
MDAMB231
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Aug 30 2017

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