Anti-Vimentin抗体(ab137321)
Key features and details
- Rabbit polyclonal to Vimentin
- Suitable for: WB, ICC/IF, IHC-P, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Vimentin抗体
参阅全部 Vimentin 一抗 -
描述
兔多克隆抗体to Vimentin -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-P, IPmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Chicken, Guinea pig, Cow, Dog, Chimpanzee -
免疫原
Recombinant fragment within Human Vimentin (internal sequence). The exact sequence is proprietary.
Database link: P08670 -
阳性对照
- WB: HEK-293T, HeLa and NIH/3T3 whole cell lysates. U87-MG, SK-N-SH. IMR32 and SK-N-AS whole cell extracts. ICC/IF: HeLa cells. IHC-P: Adult mouse retina tissue. U373 xenograft tissue and RT2 rat xenograft tissue. IP: HeLa whole cell extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137321于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 54 kDa.
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ICC/IF |
1/100 - 1/1000.
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IHC-P | (1) |
1/100 - 1/1000.
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IP |
Use at an assay dependent concentration.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 54 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
IP
Use at an assay dependent concentration. |
靶标
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功能
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. -
组织特异性
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. -
疾病相关
Cataract 30 -
序列相似性
Belongs to the intermediate filament family. -
结构域
The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. -
翻译后修饰
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 420519 Chicken
- Entrez Gene: 493952 Chimpanzee
- Entrez Gene: 280955 Cow
- Entrez Gene: 477991 Dog
- Entrez Gene: 100170760 Guinea pig
- Entrez Gene: 7431 Human
- Entrez Gene: 22352 Mouse
- Entrez Gene: 81818 Rat
see all -
形式
Vimentin is found in connective tissue and in the cytoskeleton. -
别名
- CTRCT30 antibody
- Epididymis luminal protein 113 antibody
- FLJ36605 antibody
see all
图片
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All lanes : Anti-Vimentin antibody (ab137321) at 1/50000 dilution
Lane 1 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell extracts
Lane 2 : SK-N-SH (Human neuroblastoma cell line) whole cell extracts
Lane 3 : IMR32 whole cell extracts
Lane 4 : SK-N-AS whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 54 kDa10% SDS-PAGE gel
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Paraffin-embedded adult mouse retina tissue stained for Vimentin with ab137321 (1/250 dilution) (green) in immunohistochemical analysis.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI. -
Paraffin-embedded rat testis tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse testis tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse ovary tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Vimentin (green) using ab137321 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue).
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Vimentin was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts using 5 µg of ab137321.
Lane 1: HeLa whole cell extracts (Input).
Lane 2: Control IgG in HeLa whole cell extracts.
Lane 3: Vimentin IP in HeLa whole cell extracts.
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Paraffin-embedded human lung carcinoma tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.
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Immunohistochemical analysis of paraffin-embedded, formalin-fixed RT2 rat xenograft tissue labeling Vimentin, using ab137321 at a 1/500 dilution.
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Anti-Vimentin antibody (ab137321) at 1/3000 dilution + HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Predicted band size: 54 kDa7.5% SDS PAGE
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Confocal immunofluorescence analysis of methanol-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Vimentin, using ab137321 (green) at a 1/500 dilution. Alpha-tubulin filaments were labelled with an anti-alpha tubilin antibody (red) at a 1/2000 dilution.
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All lanes : Anti-Vimentin antibody (ab137321) at 1/3000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg
Predicted band size: 54 kDa10% SDS PAGE
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Anti-Vimentin antibody (ab137321) at 1/1000 dilution + NIH-3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 54 kDa
10% SDS Page -
Immunohistochemical analysis of paraffin-embedded, formalin-fixed U373 xenograft tissue labeling Vimentin, using ab137321 at a 1/500 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (224)
ab137321 被引用在 224 文献中.
- Zheng P et al. Circ_SATB2 knockdown inhibits the tumorigenesis of non-small cell lung cancer via miR-760/KIF2A axis. Histol Histopathol 38:431-441 (2023). PubMed: 36196919
- Tu J et al. PU.1 promotes development of rheumatoid arthritis via repressing FLT3 in macrophages and fibroblast-like synoviocytes. Ann Rheum Dis 82:198-211 (2023). PubMed: 36198439
- Ni Q et al. Exosomal microRNA-23a-3p contributes to the progression of cholangiocarcinoma by interaction with Dynamin3. Bioengineered 13:6208-6221 (2022). PubMed: 35200104
- Shi Y et al. NCAPG facilitates colorectal cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition by activating the Wnt/β-catenin signaling pathway. Cancer Cell Int 22:119 (2022). PubMed: 35292013
- Wang Y et al. Circ_0005576 Exerts an Oncogenic Role in Cervical Cancer via miR-1305-Dependent Regulation of PAIP1. Reprod Sci 29:2647-2658 (2022). PubMed: 35378711