Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (small intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6
Permeabilization
Yes - 5 min in PBST (0.05% Tween 20)
Specification
small intestine
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
19 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA/PBS
Dilution
1/100
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: AF488 Donkey anti Rabbit min cross reactivity
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Abcam response
Thank you for participating in our AbTrial program and for your valuable feedback. We are pleased that this antibody worked well for you on chicken samples; this antibody is predicted to work with chicken, and we have not tested it yet in this species. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Tal Melkman-Zehavi
Verified customer
提交于 Feb 26 2020