Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Intestinal organoids)
Permeabilization
Yes - Triton X-100 0.5% in 1X PBS pH 7.4
Specification
Intestinal organoids
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: blocking buffer
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary A
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Mahi Rahman
Verified customer
提交于 May 26 2021