重组Anti-VGLUT2抗体[EPR21085] (ab216463)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21085] to VGLUT2
- Suitable for: ICC/IF, WB, IHC-P, IHC-Fr, IP
- Reacts with: Mouse, Rat
Related conjugates and formulations
概述
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产品名称
Anti-VGLUT2抗体[EPR21085]
参阅全部 VGLUT2 一抗 -
描述
兔单克隆抗体[EPR21085] to VGLUT2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-P, IHC-Fr, IPmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse cerebral cortex, hypothalamus, hippocampus and brain lysates; Rat brain, cortex and cerebellum lysates; IHC-P: Mouse brain and cerebrum tissues; Rat brain tissue; IHC-Fr: Mouse thalamus tissue; Rat cortex tissue; IP: Mouse brain lysate; ICC/IF: Rat and rat midbrain neuron cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR21085 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab216463于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/250.
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WB |
1/1000. Predicted molecular weight: 64 kDa.
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr |
Use at an assay dependent concentration.
Use at 1/500 dilution for mouse and at 1/100 dilution for rat. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP |
1/30.
|
说明 |
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ICC/IF
1/250. |
WB
1/1000. Predicted molecular weight: 64 kDa. |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
Use at an assay dependent concentration. Use at 1/500 dilution for mouse and at 1/100 dilution for rat. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/30. |
靶标
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功能
Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. -
组织特异性
Predominantly expressed in adult brain. Expressed in amygdala, caudate nucleus, cerebral cortex, frontal lobe, hippocampus, medulla, occipital lobe, putamen, spinal cord, substantia nigra, subthalamic nucleus, temporal lobe and thalamus. -
序列相似性
Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily. -
发展阶段
Expressed in fetal brain. -
细胞定位
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome. - Information by UniProt
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数据库链接
- Entrez Gene: 140919 Mouse
- Entrez Gene: 84487 Rat
- SwissProt: Q8BLE7 Mouse
- SwissProt: Q9JI12 Rat
- Unigene: 256618 Mouse
- Unigene: 19372 Rat
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别名
- Differentiation associated BNPI antibody
- Differentiation associated Na dependent inorganic phosphate cotransporter antibody
- Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody
see all
图片
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse midbrain neuron cells labelling VGLUT2 with ab216463 at 1/250 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining in mouse midbrain neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).
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Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling VGLUT2 with ab216463 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granularly cytoplasmic staining in mouse cerebrum is observed (PMID: 24804702). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen sections of mouse thalamus tissue labeling VGLUT2 with ab216463 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining in mouse thalamus.
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat midbrain neuron cells labelling VGLUT2 with ab216463 at 1/250 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining in rat midbrain neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).
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All lanes : Anti-VGLUT2 antibody [EPR21085] (ab216463) at 1/1000 dilution
Lane 1 : Mouse cerebral cortex lysate
Lane 2 : Mouse hypothalamus lysate
Lane 3 : Mouse spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 64 kDa
Observed band size: 64 kDa
Exposure time: 24 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Target is expressed in neurons, with spleen acting as a negative control (PMID: 15682395, PMID: 15102489). Note that the lysates were not boiled prior to gel loading to avoid aggregation of this membrane protein. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
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VGLUT2 was immunoprecipitated from 0.35 mg of mouse brain lysate with ab216463 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216463 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Lane 1: Mouse brain lysate 10 µg (Input).
Lane 2: ab216463 IP in mouse brain lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216463 in mouse brain lysate.
Exposure time: 10 seconds.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling VGLUT2 with ab216463 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse forebrain, including cerebral cortex and diencephalon is observed (PMID: 16217795). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling VGLUT2 with ab216463 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat forebrain including cerebral cortex and diencephalon is observed (PMID: 16217795). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen sections of rat cortex tissue labeling VGLUT2 with ab216463 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining in rat cortex.
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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All lanes : Anti-VGLUT2 antibody [EPR21085] (ab216463) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat cortex lysate
Lane 3 : Rat cerebellum lysate
Lane 4 : Mouse brain lysate
Lane 5 : Mouse hippocampus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 64 kDa
Observed band size: 64 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Note that the lysates were not boiled prior to gel loading to avoid aggregation of this membrane protein. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (5)
ab216463 被引用在 5 文献中.
- Spahic H et al. Dysregulation of ErbB4 Signaling Pathway in the Dorsal Hippocampus after Neonatal Hypoxia-Ischemia and Late Deficits in PV+ Interneurons, Synaptic Plasticity and Working Memory. Int J Mol Sci 24:N/A (2022). PubMed: 36613949
- Jin Y et al. Long noncoding RNA PM maintains cerebellar synaptic integrity and Cbln1 activation via Pax6/Mll1-mediated H3K4me3. PLoS Biol 19:e3001297 (2021). PubMed: 34111112
- Zhang H et al. Regional metabolic differences in rat prefrontal cortex measured with in vivo 1 H-MRS correlate with regional histochemical differences. NMR Biomed 32:e4024 (2019). PubMed: 30376204
- Melief EJ et al. Loss of glutamate signaling from the thalamus to dorsal striatum impairs motor function and slows the execution of learned behaviors. NPJ Parkinsons Dis 4:23 (2018). PubMed: 30083593
- Lizarbe B et al. Neurochemical Modifications in the Hippocampus, Cortex and Hypothalamus of Mice Exposed to Long-Term High-Fat Diet. Front Neurosci 12:985 (2018). PubMed: 30670942