Application
Western blot
Sample
Mouse Tissue lysate - whole (Retina)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
4 µg
Specification
Retina
Blocking step
Protein free T20 blocking solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 23 kDa Non-specific: 45, 35, 17 kDa
Additional data
Additional Notes
Three Mouse retina were homogenized in RIPA including protease inhibitor cocktail. Each protein aliquots were separated by 10% SDS-PAGE (lane 1, 2, and 3) and transferred onto 0.45 um PVDF membrane. Immunoreactivity of HRP-conjugated IgG was detected by Clarity western ECL substarte within 2 min.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 30 2018