Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (MDA-MD-231 cells injected in mouse mammary fat pad)
Specification
MDA-MD-231 cells injected in mouse mammary fat pad
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
Yes - Tween in PBS
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST + 1% BSA
Secondary antibody
Name
Non-Abcam antibody was used: Vector Lab Biotinylated Anti-Mouse IgG BA-9200
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
counter stained with Hematoxylin 1:10 dilution for 30 sec. room temp. Rinsed with H20
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提交于 Jun 18 2010