Anti-VEGF Receptor 2 (phospho Y1054 + Y1059)抗体(ab5473)
Key features and details
- Rabbit polyclonal to VEGF Receptor 2 (phospho Y1054 + Y1059)
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-VEGF Receptor 2 (phospho Y1054 + Y1059)抗体
参阅全部 VEGF Receptor 2 一抗 -
描述
兔多克隆抗体to VEGF Receptor 2 (phospho Y1054 + Y1059) -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic phospho peptide (Human)containing Tyrosines 1054 and 1059. This sequence is conserved in mouse and rat.
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阳性对照
- IF: VEGF treated HUVEC whole cells. WB: VEGF treated HUVEC, MDA-MB-231 and MCF7 whole cell extract.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA, 50% Glycerol -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated VEGFR 2. The final product is generated by affinity chromatography using a VEGFR 2 derived peptide that is phosphorylated at Tyrosines 1054 and 1059. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab5473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/250. | |
| WB | Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 200 kDa. |
靶标
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功能
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
疾病相关
Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3791 Human
- Entrez Gene: 16542 Mouse
- Entrez Gene: 25589 Rat
- Omim: 191306 Human
- SwissProt: P35968 Human
- SwissProt: P35918 Mouse
- SwissProt: O08775 Rat
- Unigene: 479756 Human
see all -
别名
- CD309 antibody
- CD309 antigen antibody
- EC 2.7.10.1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 (phospho Y1054 + Y1059) antibody (ab5473)Immunofluorescence analysis of HUVEC cells labelling VEGF Receptor 2 (phospho Y1054 + Y1059) (PAnel a: green) using ab5473 at 2 ug/ml in 0.1% BSA for 3 hours at room temperature, follwed by Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilution. Pbel b: Nuclei stained with DAPI (blue), Panel c: F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin (red), Panel d: Merged images. The images were captured at 60X magnification.
Prior antibody incubation, HUVEC (Human umbilical vein endothelial cell line) cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at room temperature, followed by treatment with 100 ng of VEGF for 30 minutes. Assay was done on 90% confluent log phase HUVEC cells.
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Western blot - Anti-VEGF Receptor 2 (phospho Y1054 + Y1059) antibody (ab5473)All lanes : Anti-VEGF Receptor 2 (phospho Y1054 + Y1059) antibody (ab5473) at 1/1000 dilution
Lane 1 : HUVEC (Human umbilical vein endothelial cell line) whole cell extract with Skimmed milk
Lane 2 : HUVEC cells treated with 25ng/ml VEGF for 5 minutes with Skimmed milk
Lane 3 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell extract with Skimmed milk
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell extract with Skimmed milk
Lysates/proteins at 30 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/5000 dilutionA 130 kDa band corresponding to VEGFR (pYpY1054/1059) was observed across cell lines tested.
Protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard.
Resolved proteins were then transferred onto a nitrocellulose membrane by over night wet transfer.
Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.
实验方案
文献 (25)
ab5473 被引用在 25 文献中.
- Li L et al. Brivanib, a multitargeted small-molecule tyrosine kinase inhibitor, suppresses laser-induced CNV in a mouse model of neovascular AMD. J Cell Physiol 235:1259-1273 (2020). PubMed: 31270802
- Cho YR et al. A novel role for a-viniferin in suppressing angiogenesis by blocking the VEGFR-2/p70S6K signaling pathway. Phytother Res N/A:N/A (2020). PubMed: 32400050
- Zhang Q et al. Acidic Bile Salts Induce Epithelial to Mesenchymal Transition via VEGF Signaling in Non-Neoplastic Barrett's Cells. Gastroenterology 156:130-144.e10 (2019). PubMed: 30268789
- Zhang Q et al. Improved Antitumor Efficacy of Combined Vaccine Based on the Induced HUVECs and DC-CT26 Against Colorectal Carcinoma. Cells 8:N/A (2019). PubMed: 31121964
- Gao Z et al. Apatinib enhanced anti-tumor activity of cisplatin on triple-negative breast cancer through inhibition of VEGFR-2. Pathol Res Pract 215:152422 (2019). PubMed: 31079851
