Application
Western blot
Sample
Human Cell lysate - whole cell (human umbilical vein endothelial cell)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10%)
Loading amount
20 µg
Treatment
siRNA for 48h
Specification
human umbilical vein endothelial cell
Blocking step
Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: ~25°C
Other product details
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: peroxidase-conjuated goat anti-rabbit lgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Other
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Other
Detection
Detection method
ECL+
Exposure
8 second(s)
Bands
Specific: 130kDa kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 02 2017