Anti-VDAC1 / Porin抗体[20B12AF2] (ab14734)
![Western blot - Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734)](https://www.abcam.cn/ps/products/14/ab14734/Images/ab14734-15-anti-vdac1-porin-antibody-20b12af2-western-blot-human-lysate.jpg "Western blot - Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734)")
Key features and details
- Mouse monoclonal [20B12AF2] to VDAC1 / Porin
- Suitable for: WB, ICC/IF, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2b
概述
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产品名称
Anti-VDAC1 / Porin抗体[20B12AF2]
参阅全部 VDAC1 / Porin 一抗 -
描述
小鼠单克隆抗体[20B12AF2] to VDAC1 / Porin -
宿主
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB MouseHuman
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免疫原
Recombinant full length protein. This information is considered to be commercially sensitive.
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阳性对照
- WB: Isolated mitochondria from human, cow, rat and mouse heart. HepG2 cell lysate. ICC/IF: HeLa cells. Human fibroblasts. Flow Cyt: HepG2 cells.
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常规说明
This antibody clone [20B12AF2] is manufactured by Abcam.
If you require this antibody in a different buffer formulation or a different conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: HEPES, Sodium chloride -
Concentration information loading... -
纯度
IgG fraction -
纯化说明
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
克隆
单克隆 -
克隆编号
20B12AF2 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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KO cell pellets
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| 应用 | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC/IF |
Human
|
| WB |
Mouse
Human
|
| All applications |
Sheep
Goat
Cat
Dog
Pig
Drosophila melanogaster
Fish
Quail
Common marmoset
Dogfish
Catshark
|
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (17) |
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa.
|
| ICC/IF | (3) |
Use at an assay dependent concentration.
|
| Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa. |
|
ICC/IF
Use at an assay dependent concentration. |
|
Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis. -
组织特异性
Heart, liver and skeletal muscle. -
序列相似性
Belongs to the eukaryotic mitochondrial porin family. -
结构域
Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity. -
细胞定位
Mitochondrion outer membrane. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 282119 Cow
- Entrez Gene: 7416 Human
- Entrez Gene: 22333 Mouse
- Entrez Gene: 397010 Pig
- Entrez Gene: 83529 Rat
- Omim: 604492 Human
- SwissProt: P45879 Cow
- SwissProt: P21796 Human
see all -
别名
- hVDAC1 antibody
- mVDAC5 antibody
- OMP2 antibody
see all
图片
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Western blot - Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734)All lanes : Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734)
Lane 1 : Wild-type Hap1 cell lysate
Lane 2 : VDAC1 knockout Hap1 cell lysate
Lane 3 : Wild-type HEK-293T cell lysate
Lane 4 : VDAC1 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.Lanes 1 - 4: Merged signal (red and green). Green - ab14734 observed at 31 kDa. Red - loading control, ab181602 observed at 37 kDa.
ab14734 was shown to react with VDAC1 / Porin in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255444 (knockout cell lysate ab263839) was used. Wild-type and VDAC1 / Porin knockout samples were subjected to SDS-PAGE. ab14734 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734)](https://www.abcam.cn/ps/products/14/ab14734/Images/ab14734-320101-anti-vdac1-porin-antibody-20b12af2-western-blot.jpg)
Western blot - Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734)Li et al PLoS One. 2016 Feb 22;11(2):e0149728. doi: 10.1371/journal.pone.0149728. eCollection 2016. Fig 5.Double immunofluorescent staining and immunoblot analysis of the endogenous SCPX and SCP2 proteins in MA-10 cells.
Immunoblot analysis of endogenous SCP2 in subcellular fractions of MA-10 cell lysates. VDAC1 is used as a mitochondrial marker protein, and GAPDH is used as a whole cell lysate loading control.
Lane 1: Whole cell lysate
Lane 2: Cytosolic fraction
Lane 3: Mitochondrial-enriched fraction
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![Western blot - Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734)](https://www.abcam.cn/ps/products/14/ab14734/Images/ab14734-22081-anti-vdac1-porin-antibody-20b12af2-western-blot.jpg)
Western blot - Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734)All lanes : Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734)
Lane 1 : Isolated mitochondria from human heart at 15 µg
Lane 2 : Isolated mitochondria from bovine heart at 6 µg
Lane 3 : Isolated mitochondria from rat heart at 30 µg
Lane 4 : Isolated mitochondria from mouse heart at 30 µg
Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 30 µg
Observed band size: 37 kDa why is the actual band size different from the predicted?
Extra bands in the mouse sample (lane 4) are due to the reaction of the secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs. -
![Immunocytochemistry/ Immunofluorescence - Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734)](https://www.abcam.cn/ps/products/14/ab14734/Images/ab14734-260604-anti-vdac1-porin-antibody-20b12af2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734)This image is courtesy of an Abreview by Michiel Krols.ab14734 staining VDAC1 / Porin in human HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 for 3 minutes and blocked with 0.2% serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/200 in 0.5% BSA and 0.02% Triton X100 in PBS) for 16 hours at 4°C. An FITC-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody at a dilution of 1/200.
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![Flow Cytometry - Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734)](https://www.abcam.cn/ps/products/14/ab14734/Images/ab14734-22082-anti-vdac1-porin-antibody-20b12af2-flow-cytometry.jpg)
Flow Cytometry - Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734)Overlay histogram showing HepG2 (Human liver hepatocellular carcinoma cell line) cells stained with ab14734 (red line).
The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14734, 1 µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2 µg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events was performed.
This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
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![Immunocytochemistry/ Immunofluorescence - Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734)](https://www.abcam.cn/ps/products/14/ab14734/Images/ab14734-22085-anti-vdac1-porin-antibody-20b12af2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734)Immunofluoresence using ab14734 at 0.2 µg/ml on human fibroblasts (red).
Nuclei were labeled with DAPI (blue). -
![Western blot - Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734)](https://www.abcam.cn/ps/products/14/ab14734/Images/ab14734-256083-anti-vdac1-porin-antibody-20b12af2-western-blot.jpg)
Western blot - Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734)Image courtesy of an anonymous abreview.Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734) at 1/500 dilution + MCF7 (Human breast adenocarcinoma cell line) cell lysate at 10 µg
Secondary
HRP-conjugated goat anti-mouse polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3 minutes0.5% TBS-tween + Lait 5% NaN3 for 16 hours at 4ºC.
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![Western blot - Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734)](https://www.abcam.cn/ps/products/14/ab14734/Images/ab14734-22084-anti-vdac1-porin-antibody-20b12af2-western-blot.jpg)
Western blot - Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734)This image is courtesy of an anonymous AbreviewAll lanes : Anti-VDAC1 / Porin antibody [20B12AF2] (ab14734) at 1/5000 dilution
Lanes 1-2 : Rat brain cell lysate (homogenate)
Lanes 3-4 : Rat brain cell lysate (mitochondrial)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP conjugated sheep anti-mouse IgG
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 39 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
文献 (330)
ab14734 被引用在 330 文献中.
- Dirks ML et al. Short-term bed rest-induced insulin resistance cannot be explained by increased mitochondrial H2 O2 emission. J Physiol 598:123-137 (2020). WB ; Human . PubMed: 31721213
- Pignanelli C et al. Low-load resistance training to task failure with and without blood flow restriction: muscular functional and structural adaptations. Am J Physiol Regul Integr Comp Physiol 318:R284-R295 (2020). WB ; Human . PubMed: 31823670
- Ke Q et al. UCP2-induced hypoxia promotes lipid accumulation and tubulointerstitial fibrosis during ischemic kidney injury. Cell Death Dis 11:26 (2020). PubMed: 31932578
- Lobo-Jarne T et al. Multiple pathways coordinate assembly of human mitochondrial complex IV and stabilization of respiratory supercomplexes. EMBO J N/A:e103912 (2020). PubMed: 32511785
- Kathuria A et al. Transcriptome analysis and functional characterization of cerebral organoids in bipolar disorder. Genome Med 12:34 (2020). PubMed: 32306996
