Anti-VCAM1抗体(ab115135)
Key features and details
- Rabbit polyclonal to VCAM1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-VCAM1抗体
参阅全部 VCAM1 一抗 -
描述
兔多克隆抗体to VCAM1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat
预测可用于: Horse, Macaque monkey -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse Spleen, Rat Spleen, Mouse Thymus, Rat Thymus, and Mouse Lung tissue lysates; LADMAC and LPS treated bEnd.3 cell lysates; ICC/IF: NIH-3T3 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115135于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 81 kDa).
Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for recommended treatment conditions and positive controls. |
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ICC/IF |
Use a concentration of 5 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 81 kDa). Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for recommended treatment conditions and positive controls. |
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. -
组织特异性
Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. -
序列相似性
Contains 7 Ig-like C2-type (immunoglobulin-like) domains. -
结构域
Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion. -
翻译后修饰
Sialoglycoprotein. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 22329 Mouse
- Entrez Gene: 25361 Rat
- SwissProt: P29533 Mouse
- SwissProt: P29534 Rat
- Unigene: 440909 Mouse
- Unigene: 76649 Mouse
- Unigene: 11267 Rat
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别名
- CD106 antibody
- CD106 Antigen antibody
- INCAM 100 antibody
see all
图片
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All lanes : Anti-VCAM1 antibody (ab115135) at 1/1000 dilution
Lane 1 : LADMAC (Mouse bone marrow monocyte macrophage) whole cell lysate
Lane 2 : bEnd.3 (Mouse brain endothelioma) whole cell lysate
Lane 3 : bEnd.3 (Mouse brain endothelioma) treated with 10 µg/ml LPS for 24 h, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 81 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesRabbit monoclonal [EPR16891] to GAPDH (ab181602) used as loading control.
Blocking/Diluting buffer: 5% NFDM/TBST.
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ICC/IF image of ab115135 stained NIH-3T3 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab115135 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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All lanes : Anti-VCAM1 antibody (ab115135) at 1 µg/ml
Lane 1 : Spleen (Mouse) Tissue Lysate
Lane 2 : Spleen (Rat) Tissue Lysate
Lane 3 : Thymus (Mouse) Tissue Lysate
Lane 4 : Thymus (Rat) Tissue Lysate
Lane 5 :Mouse lung normal tissue lysate - total protein (ab29297)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 62 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
VCAM1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab115135 被引用在 3 文献中.
- Wang L et al. Danlou Tablet Activates Autophagy of Vascular Adventitial Fibroblasts Through PI3K/Akt/mTOR to Protect Cells From Damage Caused by Atherosclerosis. Front Pharmacol 12:730525 (2021). PubMed: 34867337
- Liu S et al. Shikonin Alleviates Endothelial Cell Injury Induced by ox-LDL via AMPK/Nrf2/HO-1 Signaling Pathway. Evid Based Complement Alternat Med 2021:5881321 (2021). PubMed: 34912465
- Lee JH et al. Identification of Pre-Diabetic Biomarkers in the Progression of Diabetes Mellitus. Biomedicines 10:N/A (2021). PubMed: 35052752