概述

  • 产品名称
    Anti-VCAM1抗体[6G9]
    参阅全部 VCAM1 一抗
  • 描述
    小鼠单克隆抗体[6G9] to VCAM1
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Rabbit, Human
  • 免疫原

    Purified recombinant fragment of Human VCAM1 expressed in E. Coli.

  • 阳性对照
    • HUVEC and EC cell lysate. Human lung cancer and colon cancer tissue.

性能

应用

Our Abpromise guarantee covers the use of ab98954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 81 kDa.
IHC-P 1/200 - 1/1000.
ELISA 1/10000.

靶标

  • 功能
    Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • 组织特异性
    Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • 序列相似性
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • 结构域
    Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • 翻译后修饰
    Sialoglycoprotein.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD106 antibody
    • CD106 Antigen antibody
    • INCAM 100 antibody
    • INCAM-100 antibody
    • L1CAM antibody
    • MGC99561 antibody
    • V-CAM 1 antibody
    • Vascular Cell Adhesion Molecule 1 antibody
    • Vascular cell adhesion protein 1 antibody
    • VCAM 1 antibody
    • VCAM-1 antibody
    • VCAM1 antibody
    • VCAM1_HUMAN antibody
    see all

图片

  • All lanes : Anti-VCAM1 antibody [6G9] (ab98954) at 1/500 dilution

    Lane 1 : HUVEC cell lysate
    Lane 2 : EC cell lysate

    Predicted band size: 81 kDa

  • Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue (1) and colon cancer tissue (2) using ab98954 at 1/200 dilution with DAB staining.

文献

This product has been referenced in:
  • Kotha S  et al. Microvasculature-directed thrombopoiesis in a 3D in vitro marrow microenvironment. PLoS One 13:e0195082 (2018). ICC/IF . Read more (PubMed: 29617409) »
  • Valero-Muñoz M  et al. Relevance of vascular peroxisome proliferator-activated receptor ? coactivator-1a to molecular alterations in atherosclerosis. Exp Physiol 98:999-1008 (2013). Read more (PubMed: 23335007) »
See all 4 Publications for this product

客户评价及客户问答

1-10 of 10 Q&A

Question
Answer


I have contacted the lab and will let you know what explanation they have for the 55 kDa band.

As for the reference I found in our internal notes, it says: Researchers have shown detecting a band at 43kda which is a GPI-VCAM1. http://www.pnas.org/content/90/13/5919.full.pdf+html.

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Answer

Please check the online datasheets of all antibodies. You will find the required information.

Thanks!

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Answer

Thank you very much for contacting us.


The possible rabbit specific antibodies are available as;


ICAM1: the rabbit protein sequence (http://www.uniprot.org/uniprot/Q75T02) does not show any homology with human protein (http://www.uniprot.org/uniprot/P05362) so I am sorry we do not have any antibody available that can cross react with rabbit.


VCAM 1; ab98954 is the rabbit specific antibody.


SOD; ab13498


GPX: ab21966 is a good predicted candidate to try as the immunogen sequence is quite similar to rabbit (? http://www.uniprot.org/uniprot/P07203)


TNF alpha: ab66579 is predicted to work with rabbit as it has 90% immunogen sequence homology.


CRP: ab13426 immunogen sequence is homologous to rabbit so it is predicted to react.


Lipoxygenase; there are different forms of Lipoxygenase available e.g. LOX5, LOx12, LOX15. Which one you would interested in getting? ab23678 is anti LOX12 that can cross react with rabbit.

We do not have anti IL6 rabbit cross reactive antibody as the sequence of rabbit and human aren't homologous.


Where the antibodies are predicted to react with rabbit - you can avail advantage of testing discount programme. Please click the link for more information;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount


I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

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Answer

¡Muchas gracias por la información !

Esperamos entonces tus resultados para poder completar la información de estos anticuerpos.

¡Me alegra saber que están saliendo buenos resultados!

Si tienes cualquier duda respecto a cómo publicar los resultados en nuestra web mediante los Abreviews, por favor, no dudes en contactarme de nuevo.

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Answer

Thank you very much for your interest in ab98954.

To our knowledge,ab98954 has not been experimentallytested yetin rabbitbut the immunogen shows a very high homology to rabbit.

Therefore, I can offer a discount off a future purchase if you buyab98954 now, test it inrabbit and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and testab98954 in rabbit. I will then send a discount code. This code must be issued before purchasingab98954 so please wait for my reply before ordering.

2. Purchaseab98954 either by phone, fax, or online (www.abcam.com).

3. Test it in rabbit.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifab98954 turns out to be unsuitable for rabbit, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Answer

Gracias por u respuesta.

Me alegro que hayáis conseguido optimizar los resultados de ab25072. Para mandarnos los resultados en conejo de éste y otros anticuerpos, seguid las instrucciones indicadas en el siguiente link:

https://www.abcam.com/index.html?pageconfig=resource&rid=10332&viapagetrap=abreviews

Sigo sin obtener respuesta acerca del CD36, pero espero poder contestarte entre hoy y mañana.

En cuanto al anticuerpo contra VCAM que vais a probar en conejo en WB, el código de descuento es XXXXXXX, que caduca el XX-XXXX-XXXX.

Este código es canjeable por un anticuerpo primario totalmente gratuito si se realiza la compra antes de la fecha de caducidad. Para beneficiarse de esta oferta, por favor, mándanos los resultados mediante una Abreview obtenidos al testar ab98954 en conejo. Incluye por favor en los “Additional Comments” el código indicado, para que nosotros sepamos a qué corresponde dicha publicación.

Para más información acerca de publicar un Abreview, por favor, visita la página: https://www.abcam.com/Abreviews.

Te recuerdo que publicamos resultados tanto positivos como negativos en las datasheet de los productos. El código se activará una vez que se haya publicado el Abreview, y puede canjearse mencionando el código de descuento proporcionado a la hora de hacer el pedido, bien sea por teléfono, por fax, o por email. ES IMPRESCINDIBLE INDICAR DICHO CODIGO PARA PODER BENEFICIARSE DEL DESCUENTO.

Las condiciones y términos de esta oferta las encontrarás en:

https://www.abcam.com/collaborationdiscount.

Finalmente, ¿no vais a probar también otro anticuerpo contra I CAM1? Si lo vais a hacer, recordad indicádmelo antes para que os mande el código.

Si tienes cualquier duda, por favor no dudes en contactarnos. Esperamos atentamente tus resultados y te deseamos suerte con la investigación.

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Answer

Thank you very much for your interest in ab our VCAM1 antibodies.


I have checked our IHC-P-guaranteed VCAM1 antibodies for their potential cross-reactivity with rabbit tissue, and based on the immunogen sequence similarity of 86% I may indeed recommend the mouse monoclonal ab98954.

To our knowledge, ab98954 has not been tested in rabbit but may work based on the sequence similarity. Therefore, I can offer a discount off a future purchase if you buy ab98954 now, test it in rabbit in IHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.





If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab98954. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.





2. Purchase the antibody either by phone, fax, or online (www.abcam.com).

3. Test it in rabbit.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab98954 turns out to be unsuitable for rabbit, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount

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Answer

This product is indeed out of Abpromise guarantee period and I would really encourage you to let your customer know about the guarantee period in your region. As we are retesting this product in order to determine the reason of 50 kDa band so we are happy to make an exception this time and will send a free of charge replacement to your customer. The product ab106777 will be shipped in your next shipment. Could you also let you customer know about the Abreviews? If they can submit an Abreview for ab106777 then that can help other customers also. I hope this information will be helpful. Should you have any other question please do not hesitate to cotnat me.

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Answer

Thanks you for your email. I understand customer's concerns. The predicted band size of VCAM1 is 81kDa however due to protein glycosylation the observed band size should be near 100 kDa. This is what we are observing with different antibodies and the 100 kDa band size is also published in different publications. One publication or probably others also mention seeing a band at 43kDa which they named it as VCAM1-GPI. The publication is attached below. http://www.pnas.org/content/90/13/5919.full.pdf+html To confirm the specificity of this antibody and nature of protein observed at 53kDa I have been in contact with our lab and waiting for their response. I would recommend forwarding this to your customer until I get any response from our lab. I will get back to you soon with all the details. Many thanks for your cooperation in this case.

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Question

LOT NUMBER GR22383_1 ORDER NUMBER 809462 DESCRIPTION OF THE PROBLEM Multiple bands: According to the datasheet the predicted molecular weight is 81 kDa ,but we found 3 binds between 43 kDa and 55kDa ,therefore we want to know which molecular weight should we get SAMPLE •Species: human •What’s cell line or tissue : cell line : cybrid •Cell extract or Nuclear extract: Cell extract •Purified protein or Recombinant protein: Purified protein PRIMARY ANTIBODY •Species: mouse •Reacts against: human •At what dilution(s) have you tested this antibody: 1:500 •What dilution buffer was used: TBST •Incubation time: over night •Incubation temperature: 4℃ •What washing steps were done: 4 DETECTION METHOD ECl POSITIVE AND NEGATIVE CONTROLS USED no ANTIBODY STORAGE CONDITIONS -20℃ SAMPLE PREPARATION •What lysis buffer was used: RIPA •What protease inhibitors were used: EDTA-free Roche inhibitor •What loading buffer was used: BIO RAD 4x dye •Phosphatase inhibitors : EDTA-free Roche inhibitor •Did you heat the samples: temperature and time: 95℃,10 min AMOUNT OF PROTEIN LOADED 50μg ELECTROPHORESIS/GEL CONDITIONS •Reducing or non reducing gel: Reducing •Reducing agent: SDS •Gel percentage : 7.5% •Transfer conditions: (Type of membrane, Protein transfer verified): PVDF membrane ,400mA ,120 min TRANSFER AND BLOCKING CONDITIONS •Buffer: TBST •Blocking agent: milk, BSA, serum, what percentage: 10% non-fat milk •Incubation time: 1 hour •Incubation temperature: room tempture SECONDARY ANTIBODY •Species: goat •Reacts against: mouse •At what dilution(s) have you tested this antibody: 1: 10,000 •Incubation time: 1 hour •Wash steps: 4 •Fluorochrome or enzyme conjugate: enzyme conjugate •Do you know whether the problems you are experiencing come from the secondary? no HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 4 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Reduce primary Antibody concentration : 1: 1000 and 1:5000 ADDITIONAL NOTES This customer wants to know which bean is the correct size?

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Answer

Thank you for your enquiry regarding ab98954 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. Additionally, thank you for supplying an image, as this has been very beneficial in understanding your concerns. The protocol looks fine to me however I would like to make few comments/suggestions that might help to improve your results and that you could consider trying: - Use more diluted primary antibody e.g. at 1/1500 and 1/2000. - Heating sample at 95C for 5 minutes is ideal. - Try using primary antibody for couple of hours e.g. 4 hours at 4C After having discussion with colleague from lab regrettably I have to say it may take 1-2 more weeks to retest the antibody again. I am sorry I can only advise the molecular weight specificity after getting retesting results. I will be very happy to share these results with you. I again apologize as it is taking more time than initially anticipated; I have decided to provide you a free of charge replacement with other VCAM1 antibody ab106777: https://www.abcam.com/VCAM1-antibody-ab106777.html. Please have a look at the datasheet and then confirm to me if you are interested in getting this product. I will look forward to hearing from you soon.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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