Anti-VAMP8/EDB抗体(ab6186)
Key features and details
- Rabbit polyclonal to VAMP8/EDB
- Suitable for: IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-VAMP8/EDB抗体
参阅全部 VAMP8/EDB 一抗 -
描述
兔多克隆抗体to VAMP8/EDB -
宿主
Rabbit -
特异性
Reacts specifically with the 10 kDa protein of human CaCo-2, rat PC12 and dog MDCK cell extracts. -
经测试应用
适用于: IP, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Dog
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免疫原
Synthetic peptide corresponding to Human VAMP8/EDB.
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常规说明
Previously labelled as VAMP8.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: Whole serum -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab6186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | ||
| ICC/IF |
应用说明
IF: 1/50.
IM: Use at an assay dependant dilution.
IP: Use at an assay dependant dilution.
WB: 1/200 - 1/1000.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IM: Use at an assay dependant dilution.
IP: Use at an assay dependant dilution.
WB: 1/200 - 1/1000.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes. -
组织特异性
Platelets. -
序列相似性
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 8673 Human
- Omim: 603177 Human
- SwissProt: Q9BV40 Human
- Unigene: 714302 Human
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别名
- EDB antibody
- Endobrevin antibody
- VAMP 8 antibody
see all
实验方案
数据表及文件
文献 (7)
ab6186 被引用在 7 文献中.
- Yang Y et al. Polyphenols differentially inhibit degranulation of distinct subsets of vesicles in mast cells by specific interaction with granule-type-dependent SNARE complexes. Biochem J 450:537-46 (2013). PubMed: 23252429
- Bethani I et al. Endosomal fusion upon SNARE knockdown is maintained by residual SNARE activity and enhanced docking. Traffic 10:1543-59 (2009). WB . PubMed: 19624487
- Mashima H et al. Involvement of vesicle-associated membrane protein 7 in human gastric epithelial cell vacuolation induced by Helicobacter pylori-produced VacA. Infect Immun 76:2296-303 (2008). PubMed: 18362137
- Lapierre LA et al. Characterization of immunoisolated human gastric parietal cells tubulovesicles: identification of regulators of apical recycling. Am J Physiol Gastrointest Liver Physiol 292:G1249-62 (2007). WB, IHC-P, IF ; Human . PubMed: 17255364
- Boström P et al. SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated in insulin sensitivity. Nat Cell Biol 9:1286-93 (2007). PubMed: 17922004
- Pooley RD et al. CytLEK1 is a regulator of plasma membrane recycling through its interaction with SNAP-25. Mol Biol Cell 17:3176-86 (2006). PubMed: 16672379
- Polgár J et al. Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion. Blood 100:1081-3 (2002). WB ; Human . PubMed: 12130530
