Anti-VAMP4抗体(ab108051)
Key features and details
- Rabbit polyclonal to VAMP4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-VAMP4抗体
参阅全部 VAMP4 一抗 -
描述
兔多克隆抗体to VAMP4 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
KLH conjugated synthetic peptide between internal sequence amino acids 23 - 53 of Human VAMP4 (NP_001172056.1, NP_003753.2).
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阳性对照
- WB: K562 cell line lysates; Mouse liver tissue lysates IHC-P: Human hepatocarcinoma
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab108051 is purified through a protein A column, followed by peptide affinity purification. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108051于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/100 - 1/500. Predicted molecular weight: 16 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/100 - 1/500. Predicted molecular weight: 16 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule. -
序列相似性
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain. -
细胞定位
Golgi apparatus > trans-Golgi network membrane. Associated with trans Golgi network (TGN) and newly formed immature secretory granules (ISG). Not found on the mature secretory organelles. - Information by UniProt
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数据库链接
- Entrez Gene: 8674 Human
- Entrez Gene: 53330 Mouse
- Omim: 606909 Human
- SwissProt: A2IDD8 Human
- SwissProt: O75379 Human
- SwissProt: O70480 Mouse
- Unigene: 6651 Human
- Unigene: 10699 Mouse
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别名
- VAMP 24 antibody
- VAMP 4 antibody
- VAMP-4 antibody
see all
图片
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Anti-VAMP4 antibody (ab108051) at 1/100 dilution + K562 cell line lysates at 35 µg
Predicted band size: 16 kDa -
Anti-VAMP4 antibody (ab108051) at 1/100 dilution + Mouse liver tissue lysates at 35 µg
Predicted band size: 16 kDa -
ab108051, at a 1/50 dilution, staining VAMP4 in formalin fixed, paraffin embedded Human hepatocarcinoma by Immunohistochemistry, followed by peroxidase conjugation of the secondary antibody and DAB staining.
数据表及文件
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Datasheet download
文献 (1)
ab108051 被引用在 1 文献中.
- Ivanova D et al. Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes. Sci Adv 7:N/A (2021). PubMed: 33931449