Anti-Vaccinia Virus抗体(ab35219)
Key features and details
- Rabbit polyclonal to Vaccinia Virus
- Suitable for: ELISA, IHC-Fr, WB, IHC-P, ICC/IF
- Reacts with: Vaccinia virus
- Isotype: IgG
概述
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产品名称
Anti-Vaccinia Virus抗体
参阅全部 Vaccinia Virus 一抗 -
描述
兔多克隆抗体to Vaccinia Virus -
宿主
Rabbit -
特异性
This antibody reacts with purified Virions. It does not react with uninfected cells. -
经测试应用
适用于: ELISA, IHC-Fr, WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Vaccinia virus -
免疫原
Tissue, cells or virus corresponding to Vaccinia Virus. Vaccinia virus, New York City Board of Health (NYCBOH) strain.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
This antibody is greater than 95% pure. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab35219于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
1/500 - 1/2000.
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IHC-Fr |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. PubMed: 25093734
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IHC-P | (2) |
Use at an assay dependent concentration. PubMed: 25093734
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ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 22615950
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说明 |
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ELISA
1/500 - 1/2000. |
IHC-Fr
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. PubMed: 25093734 |
IHC-P
Use at an assay dependent concentration. PubMed: 25093734 |
ICC/IF
Use at an assay dependent concentration. PubMed: 22615950 |
靶标
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相关性
Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process. -
别名
- Orthopoxvirus antibody
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (25)
ab35219 被引用在 25 文献中.
- Hill C et al. Polymer stealthing and mucin-1 retargeting for enhanced pharmacokinetics of an oncolytic vaccinia virus. Mol Ther Oncolytics 21:47-61 (2021). PubMed: 33869742
- Bahar MW et al. Mammalian expression of virus-like particles as a proof of principle for next generation polio vaccines. NPJ Vaccines 6:5 (2021). PubMed: 33420068
- Frey SE et al. Human antibody responses following vaccinia immunization using protein microarrays and correlation with cell-mediated and antibody dependent cellular cytotoxicity responses. J Infect Dis N/A:N/A (2021). PubMed: 33675226
- Mealiea D et al. Modeling oncolytic virus dynamics in the tumor microenvironment using zebrafish. Cancer Gene Ther 28:769-784 (2021). PubMed: 32647136
- Koehler H et al. Vaccinia virus E3 prevents sensing of Z-RNA to block ZBP1-dependent necroptosis. Cell Host Microbe 29:1266-1276.e5 (2021). PubMed: 34192517