重组Anti-V5 tag抗体[SV5-P-K] (ab206566)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SV5-P-K] to V5 tag
- Suitable for: ICC/IF, IHC-Fr, RIA, IP, WB, Indirect ELISA
- Reacts with: Species independent
概述
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产品名称
Anti-V5 tag抗体[SV5-P-K]
参阅全部 V5 tag 一抗 -
描述
兔单克隆抗体[SV5-P-K] to V5 tag -
宿主
Rabbit -
特异性
ab206566 recognises a 14 amino acid sequence (GKPIPNPLLGLDST) derived from the P and V proteins of the Paramyxo Virus simian virus 5. -
经测试应用
适用于: ICC/IF, IHC-Fr, RIA, IP, WB, Indirect ELISAmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide corresponding to V5 tag. Paramyxo Virus Simian Virus 5.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
SV5-P-K -
同种型
IgG -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
- Anti-V5 tag antibody [SV5-P-K] (ab206557)
- Anti-V5 tag antibody [SV5-P-K] (ab206558)
- Anti-V5 tag antibody [SV5-P-K] (ab206559)
- Anti-V5 tag antibody [SV5-P-K] (ab206560)
- Anti-V5 tag antibody [SV5-P-K] (ab206561)
- Anti-V5 tag antibody [SV5-P-K] (ab206562)
- Anti-V5 tag antibody [SV5-P-K] (ab206565)
- Anti-V5 tag antibody [SV5-P-K] (ab206571)
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab206566于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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RIA |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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Indirect ELISA |
Use at an assay dependent concentration.
|
说明 |
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ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
RIA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Indirect ELISA
Use at an assay dependent concentration. |
靶标
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相关性
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). -
别名
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
图片
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All lanes : Anti-V5 tag antibody [SV5-P-K] (ab206566) at 1/1000 dilution
Lane 1 :Recombinant E. coli Multi Tag protein (ab36791)
Lane 2 :Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530)
Lysates/proteins at 0.2 µg per lane.
Performed under reducing conditions.
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesAnti-V5 tag antibody [SV5-P-K] staining at 1/1000 dilution, shown in black. In Western blot, ab206566 was shown to bind specifically to V5 tag. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween$®$ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 3 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) at 1/50000 dilution.
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ELISA analysis of multi-tag protein labeling V5 tag using ab206566. Plate coated with 5 µg/mL of Multi-tag protein. Primary antibody loaded at 1000 ng/mL in first well and an 11 point serial dilution performed in duplicate for each antibody with last well left blank. Signal detection was performed using HRP-conjugated Goat anti-Mouse secondary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab206566 被引用在 6 文献中.
- Soltanmohammadi N et al. Somatic PMK-1/p38 signaling links environmental stress to germ cell apoptosis and heritable euploidy. Nat Commun 13:701 (2022). PubMed: 35121747
- Tong Y et al. Hypoxia-induced NFATc3 deSUMOylation enhances pancreatic carcinoma progression. Cell Death Dis 13:413 (2022). PubMed: 35484132
- Alagia A et al. Proximity Ligation Assay for Detection of R-Loop Complexes upon DNA Damage. Methods Mol Biol 2528:289-303 (2022). PubMed: 35704199
- Kikuchi A et al. Structural basis for activation of DNMT1. Nat Commun 13:7130 (2022). PubMed: 36414620
- Tan GK et al. Tgfß signaling is critical for maintenance of the tendon cell fate. Elife 9:N/A (2020). PubMed: 31961320
- Hunt R et al. A Single Synonymous Variant (c.354G>A [p.P118P]) in ADAMTS13 Confers Enhanced Specific Activity. Int J Mol Sci 20:N/A (2019). PubMed: 31731663