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Dear Tech Support Team, Kindly advise if the following antibodies https://www.abcam.com/V5-tag-antibody-ChIP-Grade-ab9116.html https://www.abcam.com/V5-tag-antibody-FITC-ab1274.html https://www.abcam.com/V5-tag-antibody-ab84164.html https://www.abcam.com/HA-tag-antibody-ab9134.html https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-550-pre-adsorbed-ab98795.html are covered by Abpromise for the immunohistostaining of Drosophila brain if the customer treats the brain in the following way: 10 min in 4% paraformaldehyde and proceeds with the staining. What is the abbreviation that fits this type of the treatment? Thanks in advance for your assistance and reply. Have a nice week,
Asked on Dec 19 2011
Thank you for contacting us. These antibodies are against tags so species is not applicable for these products. "10 min in 4% paraformaldehyde and proceeds with the staining" is not enough information that we need for any recommendations. Please ask the customer to provide following info - Are they planning to use tissue sections or cells? What type of tissue section or cells they will be using e.g. Eye, Brain etc. - Are they planning Immunohistochemistry on frozen sections or paraformaldehyde sections thereby incubating sections with acetone or ethanol or treating sections with antigen retrieval method? - Now these antibodies are against tags so you may ask how they are expressing the tag in cells or tissues. Please provide the above information then only we can provide further confirmation.
回复于 Dec 19 2011