参阅全部 Uteroglobin 一抗
经测试应用适用于: IHC-P, ELISA, ICC/IF, WB, IHC-Frmore details
种属反应性与反应: Mouse, Rat, Human
Recombinant rat uteroglobin His fusion protein.
This antibody is useful for distinguishing ciliated from non-ciliated cells in the adult mouse lung.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液Preservative: 0.01% Sodium azide
Constituent: Whole serum
Primary antibody说明This antibody is useful for distinguishing ciliated from non-ciliated cells in the adult mouse lung.
Our Abpromise guarantee covers the use of ab40873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration. PubMed: 21787397|
|ICC/IF||Use at an assay dependent concentration. PubMed: 24367567|
|WB||1/5000. Predicted molecular weight: 10 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
功能Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
组织特异性Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).
序列相似性Belongs to the secretoglobin family.
- Information by UniProt
- Blastokinin antibody
- CC10 antibody
- CC16 antibody
Adult mouse lung was embedded in paraffin and stained with ab40873 (1:1600 dilution).
ab40873 at 1/1600 dilution staining Uteroglobin in mouse embryo tissue section by Immunohistochemistry (Frozen sections). Frozen tissue slides were fixed in 4% paraformaldehyde and antigen retrieval was performed for 10 minutes in microwave oven in 0.01 M citrate buffer pH6. ab40873 stains green in proximal lung section from wild type mouse embryo as shown in image.
All lanes : Anti-Uteroglobin antibody (ab40873) at 1/5000 dilution
Lane 1 : Mouse lung whole tissue lysate with Milk
Lane 2 : Mouse kidney whole tissue lysate with Milk
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 10 kDa
Exposure time: 30 seconds
ab40873 staining Uteroglobin in human lung parenchyma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer. Samples were then blocked with 1% BSA, 0.1% Triton-X/ PBS for 15 minutes at 18°C followed by incubation with the primary antibody at a 1/500 dilution for 16 hours at 4°C. An HRP-conjugated pig anti-rabbit polyclonal was used as secondary antibody at a 1/100 dilution.
This product has been referenced in:
- Cores J et al. Safety and Efficacy of Allogeneic Lung Spheroid Cells in a Mismatched Rat Model of Pulmonary Fibrosis. Stem Cells Transl Med 6:1905-1916 (2017). ICC ; Rat . Read more (PubMed: 28783251) »
- Strupp C et al. Relationship of Metabolism and Cell Proliferation to the Mode of Action of Fluensulfone-Induced Mouse Lung Tumors. II: Additional Mechanistic Studies. Toxicol Sci 154:296-308 (2016). Read more (PubMed: 27605420) »