Anti-USP15抗体(ab4850)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP15 antibody (ab4850)

    Key features and details

    • Goat polyclonal to USP15
    • Suitable for: IHC-P
    • Reacts with: Mouse, Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-USP15抗体
      参阅全部 USP15 一抗

    • 描述

      山羊多克隆抗体to USP15

    • 宿主

      Goat

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Mouse, Human
      预测可用于: Rat, Cow, Dog, Pig

    • 免疫原

      Synthetic peptide corresponding to Human USP15 aa 970-981 (C terminal).
      Sequence:

      NDIENENCMHTN


      Database link: Q9Y4E8
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 常规说明

      Gene Ontology terms - ubiquitin-specific protease activity; cysteine-type endopeptidase activity; deubiquitination.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    应用

    Our Abpromise guarantee covers the use of ab4850 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    IHC-P Use a concentration of 2.5 - 3.8 µg/ml.

    Perform heat mediated antigen retrieval with citrate buffer (pH6).

    靶标

    • 功能

      Protease that degrades 'Lys-48'-linked polyubiquitin chains. Protects target proteins against proteasomal degradation. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.

    • 组织特异性

      Expressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary, with little or no expression in other tissues.

    • 序列相似性

      Belongs to the peptidase C19 family.
      Contains 1 DUSP domain.

    • 翻译后修饰

      Phosphorylated. Phosphorylation protects against ubiquitination and subsequent degradation by the proteasome.
      Ubiquitinated, leading to degradation by the proteasome.
    • Information by UniProt

    • 数据库链接

      see all

    • 别名

      • Deubiquitinating enzyme 15 antibody
      • Deubiquitinating enzyme antibody
      • EC 3 1 2 15 antibody
      • KIAA0529 antibody
      • MGC131982 antibody
      • MGC149838 antibody
      • MGC74854 antibody
      • Ubiquitin Carboxy terminal Hydrolase 15 antibody
      • Ubiquitin carboxyl-terminal hydrolase 15 antibody
      • Ubiquitin specific peptidase 15 antibody
      • Ubiquitin specific processing protease 15 antibody
      • Ubiquitin Specific Protease 15 antibody
      • Ubiquitin thioesterase 15 antibody
      • Ubiquitin thiolesterase 15 antibody
      • Ubiquitin-specific-processing protease 15 antibody
      • UBP 15 antibody
      • UBP15 antibody
      • UBP15_HUMAN antibody
      • Unph 2 antibody
      • UNPH 4 antibody
      • Unph-2 antibody
      • Unph2 antibody
      • Unph4 antibody
      • USP 15 antibody
      • Usp15 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP15 antibody (ab4850)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP15 antibody (ab4850)

      Immunohistochemical analysis of paraffin embedded Human Liver labeling USP15 with ab4850 at  2.5-3.8µg/ml.   The image shows patterned intracellular staining in hepatocytes.

      Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

       

    文献 (1)

    发表研究结果有使用 ab4850？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab4850 被引用在 1 文献中.

    • Zhao Y  et al. USP15 Enhances Re-epithelialization Through Deubiquitinating EIF4A1 During Cutaneous Wound Repair. Front Cell Dev Biol 8:529 (2020). PubMed: 32671073

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    Western blot abreview for Anti-USP15 antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (brain)
    Loading amount
    20 µg
    Specification
    brain
    Gel Running Conditions
    Reduced Denaturing (6.5)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

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    提交于 Feb 17 2009

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