Anti-uPA Receptor/U-PAR抗体(ab3129)

Submit a review Q&A (3)References (3)

Key features and details

  • Goat polyclonal to uPA Receptor/U-PAR
  • Suitable for: IP, Neutralising
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-uPA Receptor/U-PAR抗体
    参阅全部 uPA Receptor/U-PAR 一抗

  • 描述

    山羊多克隆抗体to uPA Receptor/U-PAR

  • 宿主

    Goat

  • 经测试应用

    适用于: IP, Neutralisingmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant full length protein corresponding to Human uPA Receptor/U-PAR.

性能

应用

Our Abpromise guarantee covers the use of ab3129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use a concentration of 5 - 10 µg/ml.
Neutralising Use a concentration of 2 - 4 µg/ml.

靶标

  • 功能

    Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.

  • 组织特异性

    Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.

  • 序列相似性

    Contains 3 UPAR/Ly6 domains.

  • 细胞定位

    Secreted and Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CD 87 antibody
      • CD87 antibody
      • CD87 antigen antibody
      • MO 3 antibody
      • MO3 antibody
      • Monocyte activation antigen Mo3 antibody
      • Plasminogen activator receptor urokinase antibody
      • Plasminogen activator urokinase receptor antibody
      • PLAUR antibody
      • U PAR antibody
      • u plasminogen activator receptor antibody
      • U-PAR antibody
      • u-plasminogen activator receptor form 2 antibody
      • UPA receptor antibody
      • uPAR antibody
      • UPAR_HUMAN antibody
      • Urinary plasminogen activator receptor antibody
      • URKR antibody
      • Urokinase plasminogen activator receptor antibody
      • Urokinase plasminogen activator surface receptor antibody
      • urokinase-type plasminogen activator (uPA) receptor antibody
      see all

    文献 (3)

    发表研究结果有使用 ab3129？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab3129 被引用在 3 文献中.

    • Pavet V  et al. Plasminogen activator urokinase expression reveals TRAIL responsiveness and supports fractional survival of cancer cells. Cell Death Dis 5:e1043 (2014). WB . PubMed: 24481457
    • Al-Souhibani N  et al. The RNA-binding zinc-finger protein tristetraprolin regulates AU-rich mRNAs involved in breast cancer-related processes. Oncogene : (2010). WB ; Human . PubMed: 20498646
    • Bezuidenhout L  et al. Ang-2 and PDGF-BB cooperatively stimulate human peripheral blood monocyte fibrinolysis. J Leukoc Biol 81:1496-503 (2007). Neutralising ; Human . PubMed: 17395886

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Question

    Thank you for kindly reply. I measured annexin v and Propidium Iodide(PI) in FACS, about 90 % cells were PI staining positive. So I considered cells were necrosis, moreover under the microscope cells are detached and morphological change occur and no fungus/bacteria contamination were obvious. According to your advice we use 0.5 ug/ml  concentration because when I used this concentration %PI positive cells was normal distribution. And I could not find answer of the last question about this product. Are there no changes in this product from 2007 to present? Sincerely yours  

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    Answer

    Thank you for your email. The lot number customer used in 2007 was different than you have been using. So we would expect some variations due to cell line or other lab related factors. I can assure you that there is no change in the antibody itself other than difference in lot. We are also not sure whether this antibody has any lot related issues, so I am sorry I am unable to comment on this. I would also like to ask is this antibody able to neutralize the effect of uPA? I hope this information will help. Should you have any other question please do not hesitate to ask.

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    Question

    Dear Sir/Madam I purchased your product  "uPA receptor antibody cat.no, ab3129". I used this antibody in order to neutralize uPA receptor, so I added this antibody into culture medium for 24 hrs. I cultured human pulmonary artery smooth muscle cells (invitrogen) in 0.5 % fcs/Medium231(invitrogen). Then I measured apoptosis using annexin v and staining method by FACS. Surprisingly when I added 2-4ug/ml concentration of this antibody, cells were almost died. So, I have a question. Is this antibody have cell toxicity component? According to reference that was mentioned in datasheet "Bezuidenhout L et al. Ang-2 and PDGF-BB cooperatively stimulate human peripheral blood monocyte fibrinolysis. J Leukoc Biol 81:1496-503 (2007)" , cells was not died. But this paper was published 2007 ,are there any changes in this product from 2007 to present? And when I use 0.5ug/ml concentration of this product, cells were alive. Can I use this concentration as blocking uPA receptor?                

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    Answer

    Thank you for contacting us. Could you specify are the cells just dying or there is some kind of fungus or bacterial growth? The antibody solution only contain sodium azide as cytotoxic compound however the affect of this would be minimal at the Abcam recommended dilutions. The recommended concentration and dilutions to use is between 2-4 ug/ml. However this is for guideline purpose only. The dilutions have to be determined by end user because the conditions vary from cells to cells. We would recommend using a range of dilution e.g. 0.5 ug/ml, 1 ug/ml and 2 ug/ml in order to get optimized neutralization in the cell line you have been using. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    Could you help recommend a standard/positive control for uPAR run on an SDS-PAGE gel? Our customer is running homogenized mouse skeletal muscle. Thanks!

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    Answer

    Thank you for your enquiry. Ab3129 has not yet been tested for cross-reactivity with mouse uPA, so I would suggest a control from human samples. For example, uPA and uPAR are known to be overexpressed in mesenchymal and epithelial tumor cells. If you have any additional questions, please contact us again.

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