重组Anti-uPA Receptor/U-PAR抗体[EPR20191] (ab218106)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20191] to uPA Receptor/U-PAR
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-uPA Receptor/U-PAR抗体[EPR20191]
参阅全部 uPA Receptor/U-PAR 一抗 -
描述
兔单克隆抗体[EPR20191] to uPA Receptor/U-PAR -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: U937 whole cell lysate treated with 200nM PMA (Phorbol-12-myristate-13-acetate) for 72 hours. IHC-P: Human ovarian cancer, lung cancer and artery tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20191 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab218106于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 35-65 kDa (predicted molecular weight: 37 kDa).
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000. Detects a band of approximately 35-65 kDa (predicted molecular weight: 37 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. -
组织特异性
Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain. -
序列相似性
Contains 3 UPAR/Ly6 domains. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5329 Human
- Omim: 173391 Human
- SwissProt: Q03405 Human
- Unigene: 466871 Human
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别名
- CD 87 antibody
- CD87 antibody
- CD87 antigen antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling uPA Receptor/U-PAR with ab218106 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Membranous staining on dissociative cancer cells in blood vessel of human ovarian cancer is observed [PMID: 25120753].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-uPA Receptor/U-PAR antibody [EPR20191] (ab218106) at 1/1000 dilution
Lane 1 : Untreated U937 (Human histiocytic lymphoma cell line) whole cell lysate
Lane 2 : U937 whole cell lysate treated with 200nM PMA (Phorbol-12-myristate-13-acetate) for 72 hours
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 37 kDa
Observed band size: 35-65 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
PMA-stimulated U937 cells express uPA Receptor/U-PAR in the range of 35kDa-60kDa. Non-stimulated U937 cells are negative. The deglycosylated form of the protein is at 35 kDa and the highly glycosylated protein is at 60kDa (PMID:24999729).
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Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling uPA Receptor/U-PAR with ab218106 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Membranous staining on the macrophages of human ovarian cancer is observed [PMID: 25575713].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling uPA Receptor/U-PAR with ab218106 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Cytoplasmic staining on the stromal cells of human lung cancer is observed [PMID: 25575713].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human artery tissue labeling uPA Receptor/U-PAR with ab218106 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Positive staining on the neutrophils in human artery is observed [PMID: 25575713].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (4)
ab218106 被引用在 4 文献中.
- Wang LJ et al. A Novel TAF-Related Signature Based on ECM Remodeling Genes Predicts Glioma Prognosis. Front Oncol 12:862723 (2022). PubMed: 35574375
- Zhang HW et al. Morphine Suppresses Liver Cancer Cell Tumor Properties In Vitro and In Vivo. Front Oncol 11:666446 (2021). PubMed: 33968773
- Ye Y et al. Prostaglandin E2 receptor 3 (EP3) signaling promotes migration of cervical cancer via urokinase-type plasminogen activator receptor (uPAR). J Cancer Res Clin Oncol 146:2189-2203 (2020). PubMed: 32488496
- Zhang XX et al. GPAA1 promotes gastric cancer progression via upregulation of GPI-anchored protein and enhancement of ERBB signalling pathway. J Exp Clin Cancer Res 38:214 (2019). PubMed: 31118109