存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Preservative: 0.02% Sodium azide
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纯化说明Purified from ascites.
Our Abpromise guarantee covers the use of ab54430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 5 µg/ml.|
功能Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion (By similarity). Functions as netrin receptor that negatively regulates vascular branching during angiogenesis. Mediates retraction of tip cell filopodia on endothelial growth cones in response to netrin (By similarity). It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand (PubMed:12598906). Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity.
组织特异性Highly expressed in brain. Also expressed at lower level in developing lung, cartilage, kidney and hematopoietic and immune tissues.
序列相似性Belongs to the unc-5 family.
Contains 1 death domain.
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 2 TSP type-1 domains.
Contains 1 ZU5 domain.
翻译后修饰Phosphorylated on cytoplasmic tyrosine residues.
Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis.
Palmitoylation is required for pro-apoptotic activity, but not for location at lipid rafts.
细胞定位Cell membrane. Membrane raft. Associated with lipid rafts.
- Information by UniProt
- 6330415E02Rik antibody
- A630020F16 antibody
- D10Bwg0792e antibody
ab54430 staining UNC5B in mouse erythrocytes by Flow Cytometry. Collected heparinised mouse blood was incubated with erythrocyte lysing solution for 5 minutes at room temperature. The cells were washed and incubated with the primary antibody, CD45 as a general marker for leukocytes (green) and F4/80 to label macrophages (blue).
ICC/IF image of ab54430 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54430, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Srikanth P et al. Convergence of independent DISC1 mutations on impaired neurite growth via decreased UNC5D expression. Transl Psychiatry 8:245 (2018). Read more (PubMed: 30410030) »
- Miloudi K et al. Truncated netrin-1 contributes to pathological vascular permeability in diabetic retinopathy. J Clin Invest 126:3006-22 (2016). Read more (PubMed: 27400127) »