Anti-UFD1L抗体(ab96648)
Key features and details
- Rabbit polyclonal to UFD1L
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-UFD1L抗体
参阅全部 UFD1L 一抗 -
描述
兔多克隆抗体to UFD1L -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Zebrafish -
免疫原
Recombinant protein fragment corresponding to a region within amino acids 1 and 277 of UFD1L (NP_005650; UniProt ID: Q92890 isoform short).
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阳性对照
- WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates ICC/IF: A431 cell IHC-P: H441 xenograft
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96648于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 35 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 35 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures. -
组织特异性
Found in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney. -
通路
Protein degradation; proteasomal ubiquitin-dependent pathway. -
序列相似性
Belongs to the UFD1 family. -
细胞定位
Nucleus. Cytoplasm > cytosol. - Information by UniProt
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数据库链接
- Entrez Gene: 7353 Human
- Entrez Gene: 22230 Mouse
- Entrez Gene: 84478 Rat
- Omim: 601754 Human
- SwissProt: Q92890 Human
- SwissProt: P70362 Mouse
- SwissProt: Q9ES53 Rat
- Unigene: 474213 Human
see all -
别名
- UB fusion protein 1 antibody
- Ubiquitin fusion degradation 1 like (yeast) antibody
- Ubiquitin fusion degradation 1 like antibody
see all
图片
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All lanes : Anti-UFD1L antibody (ab96648) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa
10% SDS PAGE -
ab96648, at a 1/200 dilution, staining UFD1L in paraformaldehyde fixed A431 by Immunofluorescence analysis.
Lower image: merged with DNA probe -
ab96648, at a 1/500 dilution, staining UFD1L in paraffin embedded H441 xenograft by Immunohistochemical analysis.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab96648 被引用在 2 文献中.
- Wrobel L et al. Compounds activating VCP D1 ATPase enhance both autophagic and proteasomal neurotoxic protein clearance. Nat Commun 13:4146 (2022). PubMed: 35842429
- Meyer C et al. The TIA1 RNA-Binding Protein Family Regulates EIF2AK2-Mediated Stress Response and Cell Cycle Progression. Mol Cell 69:622-635.e6 (2018). PubMed: 29429924