Anti-Ubiquilin/UBQLN1抗体(ab3341)
Key features and details
- Rabbit polyclonal to Ubiquilin/UBQLN1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human, Chinese hamster
- Isotype: IgG
概述
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产品名称
Anti-Ubiquilin/UBQLN1抗体
参阅全部 Ubiquilin/UBQLN1 一抗 -
描述
兔多克隆抗体to Ubiquilin/UBQLN1 -
宿主
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Chinese hamster -
免疫原
Synthetic peptide corresponding to Mouse Ubiquilin/UBQLN1 aa 2-18.
Sequence:AESAESGGPPGAQDSAA
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常规说明
This product was previously labelled as Ubiquilin
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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IHC-P |
Human
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WB |
Chinese hamster
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应用 | Ab评论 | 说明 |
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IHC-P |
1/20 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.
By Western blot, this antibody detects an ~63 kDa protein representing Ubiquilin from CHO cell extract. |
说明 |
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IHC-P
1/20 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa. By Western blot, this antibody detects an ~63 kDa protein representing Ubiquilin from CHO cell extract. |
靶标
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功能
Links CD47 to the cytoskeleton. Promotes the surface expression of GABA-A receptors (By similarity). Promotes the accumulation of uncleaved PSEN1 and PSEN2 by stimulating their biosynthesis. Has no effect on PSEN1 and PSEN2 degradation. -
组织特异性
Ubiquitous. Highly expressed throughout the brain; detected in neurons and in neuropathological lesions, such as neurofibrillary tangles and Lewy bodies. Highly expressed in heart, placenta, pancreas, lung, liver, skeletal muscle and kidney. -
序列相似性
Contains 1 UBA domain.
Contains 1 ubiquitin-like domain. -
结构域
Binds PSEN1 and PSEN2 via its UBA domain. -
翻译后修饰
Phosphorylated. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 29979 Human
- Entrez Gene: 56085 Mouse
- Omim: 605046 Human
- SwissProt: Q9UMX0 Human
- SwissProt: Q8R317 Mouse
- Unigene: 9589 Human
- Unigene: 182053 Mouse
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别名
- DA41 antibody
- DSK2 antibody
- FLJ90054 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquilin/UBQLN1 antibody (ab3341)
Immunohistochemistry analysis of Ubiquilin showing staining in the nucleus and weak staining in the cytoplasm of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3341 diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquilin/UBQLN1 antibody (ab3341)
Immunohistochemistry analysis of Ubiquilin showing staining in the cytoplasm and nucleus of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3341 diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Anti-Ubiquilin/UBQLN1 antibody (ab3341) + CHO (Chinese hamster ovary cell line) whole cell lysate
Predicted band size: 63 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquilin/UBQLN1 antibody (ab3341)
Immunohistochemistry analysis of Ubiquilin showing staining in the cytoplasm and nucleus of paraffin-embedded mouse kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3341 diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab3341 被引用在 5 文献中.
- Hu C et al. Inadequate ubiquitination-proteasome coupling contributes to myocardial ischemia-reperfusion injury. J Clin Invest 128:5294-5306 (2018). PubMed: 30204128
- Adegoke OO et al. Overexpression of Ubiquilin-1 Alleviates Alzheimer's Disease-Caused Cognitive and Motor Deficits and Reduces Amyloid-ß Accumulation in Mice. J Alzheimers Dis 59:575-590 (2017). WB . PubMed: 28598849
- González-Pérez P et al. Association of UBQLN1 mutation with Brown-Vialetto-Van Laere syndrome but not typical ALS. Neurobiol Dis 48:391-8 (2012). PubMed: 22766032
- Adusei DC et al. Early developmental alterations in GABAergic protein expression in fragile X knockout mice. Neuropharmacology 59:167-71 (2010). WB ; Mouse . PubMed: 20470805
- Hiltunen M et al. Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion. J Biol Chem 281:32240-53 (2006). ICC/IF ; Mouse . PubMed: 16945923