重组Anti-SFT抗体[EPR13000(B)] (ab176561)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13000(B)] to SFT
- Suitable for: Flow Cyt (Intra), WB, IHC-P, IP
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-SFT抗体[EPR13000(B)]
参阅全部 SFT 一抗 -
描述
兔单克隆抗体[EPR13000(B)] to SFT -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, IPmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Jurkat and Raji cell lysates, Human skeletal muscle lysate, Human thryoid gland carcinoma and prostate tissues, Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13000(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176561于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 17 kDa.
|
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
1/10 - 1/100.
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说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Predicted molecular weight: 17 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
1/10 - 1/100. |
靶标
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功能
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of CHIP, TRAF6 and TRIM63/MURF1. Ubiquitinates CHIP-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4. -
组织特异性
Ubiquitous. Up-regulated in livers of iron-overloaded patients with hereditary hemochromatosis. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the ubiquitin-conjugating enzyme family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 7321 Human
- Entrez Gene: 216080 Mouse
- Entrez Gene: 361831 Rat
- Omim: 602961 Human
- SwissProt: P51668 Human
- SwissProt: P61080 Mouse
- SwissProt: D3ZDK2 Rat
- Unigene: 129683 Human
see all -
别名
- E2(17)KB1 antibody
- SFT antibody
- Stimulator of Fe transport antibody
see all
图片
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SFT knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab176561 observed at 18 kDa. Red - loading control, ab181602, observed at 37 kDa. ab176561 was shown to recognize SFT when SFT knockout samples were used, along with additional cross-reactive bands. Wild-type and SFT knockout samples were subjected to SDS-PAGE. ab176561 and ab181602 (loading control to GAPDH) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging. -
All lanes : Anti-SFT antibody [EPR13000(B)] (ab176561) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Raji cell lysate
Lane 3 : Human skeletal muscle lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 17 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human prostate tissue labeling SFT with ab176561 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human thyroid gland carcinoma tissue labeling SFT with ab176561 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of permeabilized Jurkat cells labeling SFT (red) with ab176561 at a 1/10 dilution, or negative control rabbit IgG (green)
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Western blot analysis on immunoprecipitation pellet from Human skeletal muscle lysate labeling SFT with ab176561 at 1/10 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab176561 被引用在 4 文献中.
- Yong W et al. Sodium butyrate alleviates pre-eclampsia in pregnant rats by improving the gut microbiota and short-chain fatty acid metabolites production. J Appl Microbiol 132:1370-1383 (2022). PubMed: 34470077
- Brühl J et al. The DNA repair protein SHPRH is a nucleosome-stimulated ATPase and a nucleosome-E3 ubiquitin ligase. Epigenetics Chromatin 12:52 (2019). PubMed: 31434570
- Shi Y et al. Ube2D3 and Ube2N are essential for RIG-I-mediated MAVS aggregation in antiviral innate immunity. Nat Commun 8:15138 (2017). WB ; Human . PubMed: 28469175
- Liu XJ et al. Human cytomegalovirus IE1 downregulates Hes1 in neural progenitor cells as a potential E3 ubiquitin ligase. PLoS Pathog 13:e1006542 (2017). PubMed: 28750047