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Neuroscience Neurotransmitter Biogenic Amines Dopamine

Anti-Tyrosine Hydroxylase抗体- Neuronal Marker (ab112)

  • Datasheet
  • SDS
Reviews (34)Q&A (10)References (179)

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Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
  • Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)

Key features and details

  • Rabbit polyclonal to Tyrosine Hydroxylase - Neuronal Marker
  • Suitable for: IHC-P, WB
  • Reacts with: Rat
  • Isotype: IgG

重组抗体的批间可重复性更强

Product image
Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Tyrosine Hydroxylase抗体- Neuronal Marker
    参阅全部 Tyrosine Hydroxylase 一抗
  • 描述

    兔多克隆抗体to Tyrosine Hydroxylase - Neuronal Marker
  • 宿主

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    WB
    Rat
    See all applications and species data
  • 免疫原

    Full length SDS denatured protein (purified from pheochromocytoma) (Rat).

  • 常规说明

    ab112 can be used as a marker for dopaminergic and noradrenergic neurons.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.50
    Constituents: 0.01% BSA, 0.87% Sodium chloride, 50% Glycerol, 0.238% HEPES
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Dopamine
    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Noradrenaline
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (Biotin) (ab6720)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)
  • Recombinant Protein

    • Recombinant Mouse Tyrosine Hydroxylase protein (His tag) (ab219278)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
IHC-P
Rat
WB
Rat
All applications
Mammals
应用 Ab评论 说明
IHC-P (15)
1/750.
WB (4)
1/200. Predicted molecular weight: 60 kDa.
说明
IHC-P
1/750.
WB
1/200. Predicted molecular weight: 60 kDa.

靶标

  • 功能

    Plays an important role in the physiology of adrenergic neurons.
  • 组织特异性

    Mainly expressed in the brain and adrenal glands.
  • 通路

    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • 疾病相关

    Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • 序列相似性

    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Target information above from: UniProt accession P07101 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 25085 Rat
    • SwissProt: P04177 Rat
    • Unigene: 11082 Rat
    • 别名

      • Dystonia 14 antibody
      • DYT14 antibody
      • DYT5b antibody
      • EC 1.14.16.2 antibody
      • OTTHUMP00000011225 antibody
      • OTTHUMP00000011226 antibody
      • ple antibody
      • Protein Pale antibody
      • TH antibody
      • The antibody
      • TY3H_HUMAN antibody
      • TYH antibody
      • Tyrosine 3 hydroxylase antibody
      • Tyrosine 3 monooxygenase antibody
      • Tyrosine 3-hydroxylase antibody
      • Tyrosine 3-monooxygenase antibody
      • Tyrosine hydroxylase antibody
      see all

    图片

    • Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
      Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
      Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) at 1/200 dilution + PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

      Predicted band size: 60 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)

      Paraffin embedded sections of rat brain tissue were stained for Tyrosine Hydroxylase with ab112 at 1/5000 dilution in immunohistochemical analysis (Panel 1).

      Panel 2 shows an image in which ab112 was replaced with a Rabbit IgG1 isotype control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)This image is courtesy of an Abreview submitted by Dr Guillermo Estivill-Torrus

      ab112 at 1/500 staining rat brain tissue (ab29475) sections by IHC-P.

      The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 14 hours. A biotinylated swine anti-rabbit IgG was used as the secondary.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)This image is courtesy of an anonymous Abreview

      ab112 at 1/800 staining rat dopaminergic neuronal tissue sections (araldite resin sections) by immunohistochemistry.

      The tissue was paraformaldehyde fixed and then an antigen retrieval step was carried out (heat mediated). A biotinylated goat anti-rabbit IgG (ab6720) was used as the secondary.

      See Abreview

    • Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
      Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
      Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) at 1/200 dilution + rat caudate lysate at 10 µg

      Predicted band size: 60 kDa

    实验方案

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (179)

    发表研究结果有使用 ab112?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab112 被引用在 179 文献中.

    • Nullmeier S  et al. Glutamic acid decarboxylase 67 haplodeficiency in mice: consequences of postweaning social isolation on behavior and changes in brain neurochemical systems. Brain Struct Funct 225:1719-1742 (2020). PubMed: 32514634
    • Zhou L  et al. Colocalization of dopamine receptors in BDNF-expressing peptidergic neurons in the paraventricular nucleus of rats. J Chem Neuroanat 106:101794 (2020). PubMed: 32315740
    • You MJ  et al. Human umbilical cord-derived mesenchymal stem cells alleviate schizophrenia-relevant behaviors in amphetamine-sensitized mice by inhibiting neuroinflammation. Transl Psychiatry 10:123 (2020). PubMed: 32341334
    • Koga K  et al. Ascending noradrenergic excitation from the locus coeruleus to the anterior cingulate cortex. Mol Brain 13:49 (2020). PubMed: 32216807
    • Cao S  et al. Fast Localization and Sectioning of Mouse Locus Coeruleus. Biomed Res Int 2020:4860735 (2020). PubMed: 32190666
    View all Publications for this product

    客户评价及客户问答

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    1-10 of 44 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (embryonic)
    Permeabilization
    Yes - Tritonx100
    Specification
    embryonic
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 05 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Dopaminergic neuron)
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    Dopaminergic neuron
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 02 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Pig Tissue sections (Heart)
    Permeabilization
    Yes - Triton
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 25 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Monkey Tissue sections (brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - Triton 0.5%
    Specification
    brain
    Blocking step
    Serum as blocking agent for 48 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Elena Borra

    Verified customer

    提交于 Oct 15 2019

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (brainstem)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    brainstem
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 07 2018

    Western blot abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Sheep Tissue lysate - whole (Adipose fat)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    20 µg
    Specification
    Adipose fat
    Blocking step
    Odyssey Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
    Read More

    Kanchan Kaushal

    Verified customer

    提交于 Sep 06 2018

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    Enzymatic
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 29 2018

    Western blot abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    20 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 28 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 May 16 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (medulla oblongata)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
    Permeabilization
    No
    Specification
    medulla oblongata
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 03 2018

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